PEG6000胁迫对大麦保护性酶活性的影响

北方农业学报 ›› 2015, Vol. ›› Issue (1): 8-8.

PEG6000胁迫对大麦保护性酶活性的影响

包海柱[1] 徐寿军[2] 张凤英[1] 刘志萍[1] 高瑞晓[3]

[1]内蒙古农牧业科学院,内蒙古呼和浩特010031 [2]内蒙古民族大学,内蒙古通辽010045 [3]呼和浩特市蔬菜技术推广站,内蒙古呼和浩特010020

出版日期:2015-02-20 发布日期:2015-02-20
通讯作者: 包海柱
作者简介:包海柱（1972-），男（蒙古族），内蒙古通辽人，副研究员，博士，主要从事大麦遗传育种工作。
基金资助:
内蒙古农牧业科学院创新基金; 国家大麦青稞产业技术体系（CARS-05）; 内蒙古特色杂粮应用研究与开发

Effect of PEG6000 stress on the protective enzyme activity of barley

BAO Haizhu,XU Shoujun,ZHANG Fengying,LIU Zhiping,GAO Ruixiao （ 1.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot OlO031,China; 2.Inner Mongolia University for Nationalities,Tongliao 028000,China; 3.Hohhot Vegetable Technology Extending Stations,Hohhot 010020,China）

Online:2015-02-20 Published:2015-02-20

摘要/Abstract

摘要： 采用聚乙二醇（PEG6000）渗透胁迫的方法,以抗旱品系13PJ-118和非抗旱品系13PJ-108为试验材料,对幼苗进行了模拟干旱胁迫,测定了胁迫条件下幼苗叶片保护性酶的活性。结果表明,在0%-25%的聚乙二醇胁迫下,随PEG浓度升高,大麦叶片SOD活性呈逐渐上升趋势,POD酶、CAT酶活性呈先上升而后下降趋势;SOD酶活性可作为首选抗旱鉴定指标,POD酶、CAT酶活性可以作为抗旱鉴定的参考生理指标。

Abstract: Simulating water stress with PEG-6000,the barley seedlings of ＂13PJ-118＂（drought tolerant） and ＂13PJ-108＂（drought sensitive） were treated with different concentrations of PEG-6000（5%,10%,15%,20%,25%）,and the activity of protective enzyme were determinated such as leaf SOD activity,POD activity,CAT activity.The results showed that the activity of SOD showed an increasing trend by the incressing of PEG-6000 concentration,but the activity of POD and CAT first increased and then decreased;The SOD activity could be used as the most important physiological index of drought resistance,and the POD activity,the CAT activity may be the major index.

中图分类号:

S562.2

包海柱徐寿军张凤英刘志萍高瑞晓. PEG6000胁迫对大麦保护性酶活性的影响[J]. 北方农业学报, 2015, (1): 8-8.