Abstract: Wood of Shinyleaf Yellowhorn is a great development value of edible oil and the representative of bioenergy suitable tree species in north of China.It has a strong ability to heat and cold resistance, strong vitality,It is a good species of sand-fixation wind.With the development of high value.Because lack of provenance and influenced in the development process, In order to expand the provenance and further perfecting Wood of Shinyleaf Yellowhorn cultivation technology.Young stem segment of Wood of Shinyleaf Yellowhorn was used as explant materials in this test,Disinfected after vaccination in different combination hormone levels on the MS culture medium of the induced rootstocks.The result showed： ①Young stem segment had induced the highest rate in MS＋6-BA1.0＋NAA0.5 medium. ②MS＋6-BA1.0＋NAA0.5 medium of differentiation coefficient was the highest in different formula transgenerational medium,and growing conditions of seedlings was the best.③rooting rate of 1/2 MS＋IBA0.5＋NAA0.05 culture medium was the highest in different concentrations of auxin,and the rooting time is shortest,root system was developed,grew well.④In transplanting experiment,matrix （peat,vermiculite,perlite） ratio of 2∶1∶1 transplanting seedlings rooting time was short,to take root the highest rate （97.2%）,the survival rate reached 80.38%and growth in good condition.