Abstract: In order to study the effect of different factors on Dianthus Linn tissue culture and rapid propagation, we had designed a series of culture medium which containing different concentration proportions of growth hormone（NAA） and cytokinin（6-BA,KT）, different specious and concentration of carbon source（sugar,white granulated sugar and maltose）,different major element（MS,1/2MS,1/4MS and 2MS） and different supplement（ Lactalbumin Hydrolysate,glutamine,coconut juice and activated carbon） to culture it＇s isolated stem segments. The results showed that when the concentration of NAA and 6-BA was 0.1mg/L/1.0mg/L, the medium suitable for explants＇ rapid propagation, while KT had significant difference in plantlet growth and root elongation. In plant tissue culture and rapid propagation, the best carbon source was sugar of 15 g/L and also would be replaced by sugar of 30 g/L to cost reduction. MS medium was fit for micro shoot multiplication, and at the same time, in the experiment the bud＇s multiplication coefficient was reach up to 9.4 after adding glutamine, which was significant than other supplements.