北方农业学报 ›› 2016, Vol. 44 ›› Issue (4): 53-53.

• 研究报告 • 上一篇    下一篇

苜蓿根瘤菌的发酵工艺优化

闫洪雪[1];刘露[1];李丽[1];赵宏涛[2];张鹏鹏[1];梁文辉[2]   

  1. [1]青岛明月海藻集团有限公司,海藻活性物质国家重点实验室,山东青岛266400 [2]青岛明月蓝海生物科技有限公司,山东青岛266400
  • 出版日期:2016-08-20 发布日期:2016-08-20
  • 通讯作者: 闫洪雪
  • 作者简介:闫洪雪(1988-),女,工程师,硕士,主要从事微生物菌剂、菌肥的研发工作。
  • 基金资助:
    国家科技部星火计划(2010GA741050)

Optimization of the fermentation conditions of Alfalfa Rhizobia

YAN Hongxue;LIU Lu;LI Li;ZHAO Hongtao;ZHANG Pengpeng;LIANG Wenhui( 1.State Key Laboratory of Bioactive Seaweed Substances Qingdao Brightmoon Seaweed Group Co. Ltd, Qingdao 266400, China ; 2.Qingdao Mingyue Blue Ocean Bio-technology Co. Ltd.,Qingdao 266400, China)   

  • Online:2016-08-20 Published:2016-08-20

摘要: 试验对1株苜蓿根瘤菌M2进行了培养条件及发酵配方的优化。通过设置不同的温度、pH值、转速,并调整发酵配方,得到最适发酵工艺:最适温度为30℃,最适pH值为灭菌前7.5,最适转速为180 r/min。经进一步调整配方后,菌数可稳定在90×10~8 cfu/m L。

Abstract: This research carried out the culture condition s and fermentation formula optimization of alfalfa rhizobia M2.By setting the different temperature, pH, speed, and adjusting the fermentation formula, we got the optimal fermentation process.The optimal temperature was 30 ℃, the optimum pH was 7.5 before sterilization, the optimal rotate speed was 180 r/min.After further formula adjustment,the bacterium number was stable at 90×10~8cfu/m L.

中图分类号: 

  • S541.9 Q939.114