Abstract: CRISPR/Cas9 is a rapidly developing genome editing technology that has been successfully applied in many organisms.Cas9,a RNA-guided DNA endonuclease,can be targeted to specific genomic sequences by engineering a separately encoded guide RNA with which it forms a complex.It can be employed to achieve precise gene modifications by gene mutations,insertions,replacements or chromosome rearrangements.CRISPR/Cas9 is a relatively cheaper and easier to implement technology that has proven to be extremely versatile.Remarkably,in some plant species,homozygous knockout mutants can be produced in a single generation.The mechanism of action,technical route,research examples of CRISPR/Cas9 were illustrated in this paper,and it will provide reference for researching in agricultural field.