北方农业学报 ›› 2018, Vol. 46 ›› Issue (4): 87-87.

• • 上一篇    下一篇

三抗体夹心酶联试剂盒中南芥菜花叶病毒阳性对照替代物研制

赵世恒[1];高润蕾[2];张光[3];李雪娇[4];云晓鹏[5]   

  1. [1]北京市通州区农产品质量检验检测站,北京100024;[2]呼和浩特市和林格尔盛乐经济园区管委会,内蒙古和林格尔011500;[3]乌拉特前旗农业技术推广中心,内蒙古乌拉山镇015000;[4]北京市通州区植物保护站,北京100024;[5]内蒙古农牧业科学院植物保护研究所,内蒙古呼和浩特010031
  • 出版日期:2018-08-20 发布日期:2018-08-20
  • 通讯作者: 赵世恒
  • 作者简介:赵世恒(1980-),男,农艺师,硕士,主要从事农产品检测以及植保研究工作。;通讯作者:云晓鹏(1981-),男,研究员,硕士,主要从事作物病虫草害检疫、防控技术研究及应用推广工作。
  • 基金资助:
    农业农村部呼和浩特作物有害生物科学观测站项目

Development of a positive control alternative in TAS-ELISA kit of Arabis mosaic virus

ZHAO Shiheng1, GAO Runlei2, ZHANG Guang3, LI Xuejiao4, YUN Xiaopeng5(1.Beijing Tongzhou District Agrieuhural Product Quality- Inspection Station, Beijing 100024, China; 2. Administrative Committee of Horinger Shengle Economic Development Zone, Horinger 011500,China; 3. Agrieuhural Technology Extension Center of Urad Front Banner, Walashan 015000,China; 4. Plant Protection Station of Tongzhou District, Beijing 100024,China;5.Plant Protection Institute, Inner Mongolia Academy of Agrieuhural & Animal Husbandry Seienees,Hohhot 010031, China)   

  • Online:2018-08-20 Published:2018-08-20

摘要: 在研制的南芥菜花叶病毒三抗体夹心酶联(TAS-ELISA)检测试剂盒中,山羊抗鼠多克隆抗体能够起到很好的阳性对照作用,具有替代侵染性的南芥菜花叶病毒的功能,可以监控整个酶联反应体系是否正常工作,避免了检疫性南芥菜花叶病毒扩散到环境中去,通过分子生物学手段验证了研究的可行性.

Abstract: We have developed a TAS-ELISA kit for detecting Arabis mosaic virus (ArMV). In order to avoid the dispersion of ArMV to the environment,a goat anti-mice polyelonal antibody in the kit was used as positive control ahernative for ArMV to monitor the whole ELISA reaction system. Finally, the feasibility of this study is verified by molecular biology.

中图分类号: 

  • S432.41