初始pH对Bacillus sp．DS3降解纤维素活性的影响

北方农业学报 ›› 2009, Vol. ›› Issue (1): 34-34.

初始pH对Bacillus sp．DS3降解纤维素活性的影响

杨钦忠[1] 李艳玉[2] 冯福应[3]

[1]内蒙古种星种业公司,内蒙古呼和浩特010018 [2]赤峰市喀喇沁旗扶贫开发办,内蒙古赤峰024400 [3]内蒙古农业大学生物工程学院．内蒙古呼和浩特010018

出版日期:2009-02-20 发布日期:2009-02-20
通讯作者: 杨钦忠
作者简介:通讯作者：冯福应（1973-），男，内蒙古包头人，副教授，博士，研究方向为环境微生物学。

The Effect of Initial pH on Bacillus sp. DS3 Cellulose Degradation Activit

YANG Qin-zhong （Zhongxin Seeds Company, Inner Mongolia, Huhhot 010018,China）

Online:2009-02-20 Published:2009-02-20

摘要/Abstract

摘要： 初始pH处理对Bacillus sp．DS3的生长、纤维素降解活性和相对纤维素降解活性影响显著。在碱性（pH8．0）条件下，DS3生长较中性（pH7．0）和酸性（pH6．0）条件下慢，到达稳定期时的细胞最高浓度明显低；但是，碱性条件下的降解活性和相对降解活性均明显高于其他pH条件。这些结果表明，DS3纤维素酶的活性最高或产酶量最大的pH条件为碱性，应用DS3于纤维素酶生产或含高纤维饲料的处理应该优化初始pH为8．0左右。

Abstract: Initial pH treatments had evident effects on the growth,cellulose-degrading activity and relative cellulose-degrading activity of Bacillus sp.DS3.The alkaline （pH8.0） favored the growth of Bacillus sp.DS3,and had the highest relative cell density in steady state than other pH treatments.But the pH appeared significant higher cellulose-degrading activity and relative cellulose-degrading activity than those of pH 7.0 and pH 6.0.The results showed that the cellulase activity or production of Bacillus sp.DS3 would be achieved the highest under alkaline,and therefore the initial pH should be about pH 8.0 when DS3 was used to produce cellulase and treat forage high-cellulose-contained.

中图分类号:

Q939.97

杨钦忠李艳玉冯福应. 初始pH对Bacillus sp．DS3降解纤维素活性的影响[J]. 北方农业学报, 2009, (1): 34-34.