苹果轮纹病菌(Botryosphaeria dothidea)拮抗细菌的筛选与鉴定

doi:10.3969/j.issn.2096-1197.2019.02.14

北方农业学报 ›› 2019, Vol. 47 ›› Issue (2): 83-87.doi: 10.3969/j.issn.2096-1197.2019.02.14

苹果轮纹病菌(Botryosphaeria dothidea)拮抗细菌的筛选与鉴定

孙圣[1];冯福应[1];张子义[1]

[1]内蒙古农业大学生命科学学院,内蒙古呼和浩特010018

收稿日期:2019-03-14 出版日期:2019-02-19 发布日期:2019-08-19
作者简介:孙圣(1992—),男,硕士,研究方向为应用与环境微生物;通讯作者:张子义(1973-),男,教授,博士,主要从事植物学的教学与研究工作。
基金资助:
内蒙古自治区科技创新引导项目.

Screening and identification of antagonistic bacteria against Botryosphaeria dothidea

SUN Sheng[1];FENG Fuying[1];ZHANG Ziyi[1]

[1]College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China

Received:2019-03-14 Online:2019-02-19 Published:2019-08-19

摘要/Abstract

摘要： 苹果轮纹病是苹果的主要病害之一。为了获得防治苹果轮纹病菌(Botryosphaeria dothidea)的生防细菌,该研究采用平板对峙法对供试的70株细菌进行筛选,初筛结果表明:共有18株供试菌株对苹果轮纹病有不同程度的抑制作用。通过计算抑菌率发现,4株细菌BS-1、B16、B169和1301的抑菌率在50.00%以上,其中B16的抑菌率最高,达到59.30%。经鉴定,BS-1为蜡样芽孢杆菌(Bacillus cereus)、B16、B169为莫海威芽孢杆菌(Bacillus mojavensis)、1301为多黏类芽孢杆菌(Paenibacillus polymyxa)。该研究表明,BS-1、B16、B169和1301对苹果轮纹病的防治具有一定的应用前景。

关键词: Botryosphaeria dothidea;平板对峙法;生防细菌;芽孢菌

Abstract: Apple ring rot caused by Botryosphaeria dothidea is one of the main apple diseases.In order to find biocontrol bacteria for apple ring rot,70 strains of bacteria were obtained using the plate confrontation method.Preliminary screening showed that 18 strains had different degrees of inhibition of apple ring rot.I It was found that the bacteriostasis rate of four strains (BS-1,B16,B169 and 1301) exceeded 50.0%,with strain B16 having the highest rate of 59.30%.Strains BS-1,B16,B169 and 1301 were identified as Bacillus cereus,Bacillus mojavensis,Bacillus mojavensis and Paenibacillus polymyxa,respectively.The results showed that these four strains had certain application prospects in the control of Botryosphaeria dothidea.

Key words: Botryosphaeria dothidea;Plate confrontation;Biocontrol bacteria;Bacillus

中图分类号:

S436.611

孙圣;冯福应;张子义. 苹果轮纹病菌(Botryosphaeria dothidea)拮抗细菌的筛选与鉴定[J]. 北方农业学报, 2019, 47(2): 83-87.

SUN Sheng;FENG Fuying;ZHANG Ziyi(College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China). Screening and identification of antagonistic bacteria against Botryosphaeria dothidea[J]. Journal of Northern Agriculture, 2019, 47(2): 83-87.

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苹果轮纹病菌(Botryosphaeria dothidea)拮抗细菌的筛选与鉴定

Screening and identification of antagonistic bacteria against Botryosphaeria dothidea

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