Ce3+、VC和Ac-DEVD-CHO浸种对沙葱种子抗氧化系统的影响

doi:10.12190/j.issn.2096-1197.2021.04.12

北方农业学报 ›› 2021, Vol. 49 ›› Issue (4): 76-82.doi: 10.12190/j.issn.2096-1197.2021.04.12

• 分子生物学·生理生化 • 上一篇下一篇

Ce³⁺、VC和Ac-DEVD-CHO浸种对沙葱种子抗氧化系统的影响

王佳琪¹, 李娜², 魏鹏超¹, 杨忠仁^1,3, 张东¹, 张凤兰^1,3

1.内蒙古农业大学园艺与植物保护学院/内蒙古自治区野生特有蔬菜种质资源与种质创新重点实验室,内蒙古呼和浩特 010011;
2.内蒙古自治区林业科学研究院,内蒙古呼和浩特 010010;
3.内蒙古自治区农牧业大数据研究与应用重点实验室,内蒙古呼和浩特 010019

收稿日期:2021-04-20 出版日期:2021-08-20 发布日期:2021-10-28
通讯作者: 杨忠仁（1980—）,男,副教授,博士,博士生导师,主要从事蔬菜种质资源创新与生理生态的研究工作。
作者简介:王佳琪（1995—）,女,硕士研究生,研究方向为蔬菜种质资源与种质创新。李娜（1982—）,女,助理研究员,硕士研究生,主要从事沙生蔬菜种质资源与种质创新的研究工作。王佳琪与李娜为同等贡献作者。
基金资助:
国家自然科学基金项目（31760081）; 内蒙古自治区应用技术研究与开发资金项目（2019GC237）; 内蒙古自然科学基金项目（2019MS03055）; 内蒙古自治区科技成果转化专项资金项目（2021CG0023）; 内蒙古农业大学动植物品种选育专项（YZGC2017016）

Effects of soaking with Ce³⁺,VC and Ac-DEVD-CHO on antioxidant system of Allium mongolicum Regel. seeds

WANG Jiaqi¹, LI Na², WEI Pengchao¹, YANG Zhongren^1,3, ZHANG Dong¹, ZHANG Fenglan^1,3

1. College of Horticulture and Plant Protection,Inner Mongolia Agricultural University/Inner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement,Hohhot 010011,China;
2. Inner Mongolia Academy of Forestry Sciences,Hohhot 010010,China;
3. Inner Mongolia Key Laboratory of Agricultural and Animal Husbandry Big Data Research and Application,Hohhot 010019,China

Received:2021-04-20 Online:2021-08-20 Published:2021-10-28

摘要/Abstract

摘要： 【目的】研究Ce³⁺、VC和Ac-DEVD-CHO浸种对不同贮藏年限沙葱种子抗氧化系统的影响。【方法】以贮藏3年、5年和7年的沙葱种子为试验材料,用蒸馏水（CK）、CeCl₃、VC、Ac-DEVD-CHO、VC+CeCl₃和Ac-DEVD-CHO+CeCl₃对沙葱种子进行浸种处理,测定不同处理、不同贮藏年限沙葱种子SOD、POD、CAT、APX活性以及·O₂^-产生速率、H₂O₂和MDA含量。【结果】与CK相比,CeCl₃处理显著提高了贮藏5年、7年沙葱种子的SOD活性（P<0.05）,不同浸种处理显著提高了贮藏3年沙葱种子的POD活性（P<0.05）,Ac-DEVD-CHO处理显著提高了贮藏5年、7年沙葱种子的CAT活性（P<0.05）,不同浸种处理显著提高了贮藏7年沙葱种子的APX活性（P<0.05）。与CK相比,不同浸种处理均能降低贮藏5年和7年沙葱种子的·O₂^-产生速率,其中,VC+CeCl₃和Ac-DEVD-CHO+CeCl₃处理显著降低了沙葱种子的·O₂^-产生速率（P<0.05）;VC、Ac-DEVD-CHO、VC+CeCl₃和Ac-DEVD-CHO+CeCl₃处理显著降低了贮藏5年、7年沙葱种子的H₂O₂含量（P<0.05）;CeCl₃和VC处理降低了贮藏5年和7年沙葱种子的MDA含量,但差异不显著（P>0.05）。【结论】与其他浸种处理相比,CeCl₃处理提高沙葱种子SOD活性的效果最好;从整体来看,VC和Ac-DEVD-CHO与CeCl₃共同处理对清除沙葱种子·O₂^-产生速率的效果更好。

关键词: 沙葱种子, 贮藏年限, CeCl₃, VC, Ac-DEVD-CHO, 抗氧化系统

Abstract: 【Objective】To study the effects of soaking with Ce³⁺,VC and Ac-DEVD-CHO on the antioxidant system of Allium mongolicum Regel. seeds with different storage years.【Methods】Allium mongolicum Regel. seeds stored for 3,5 and 7 years were soaked with distilled water（CK）,CeCl₃,VC,Ac-DEVD-CHO,VC+CeCl₃ and Ac-DEVD-CHO+CeCl₃. The activities of SOD,POD,CAT and APX,the production rate of ·O₂^-,and the contents of H₂O₂ and MDA of Allium mongolicum Regel. seeds with different treatments and storage years were measured.【Results】Compared with CK,CeCl₃ treatment significantly increased the SOD activity of Allium mongolicum Regel. seeds stored for 5 and 7 years（P<0.05）,different seed soaking treatments significantly increased the POD activity of Allium mongolicum Regel. seeds stored for 3 years（P<0.05）,Ac-DEVD-CHO treatment significantly increased the CAT activity of Allium mongolicum Regel. seeds stored for 5 and 7 years（P<0.05）,and different seed soaking treatments significantly increased the APX activity of Allium mongolicum Regel. seeds stored for 7 years（P<0.05）. Compared with CK,different seed soaking treatments decreased the ·O₂^- production rate of Allium mongolicum Regel. seeds stored for 5 and 7 years,among which VC+CeCl₃ and Ac-DEVD-CHO+CeCl₃ treatments significantly decreased the ·O₂^- production rate of Allium mongolicum Regel. seeds（P<0.05）. The treatments of VC,Ac-DEVD-CHO,VC+CeCl₃ and Ac-DEVD-CHO+CeCl₃ treatments significantly decreased the H₂O₂ content of Allium mongolicum Regel. seeds stored for 5 and 7 years（P<0.05）. CeCl₃ and VC treatments decreased the MDA content of Allium mongolicum Regel. seeds stored for 5 and 7 years,but no significant difference was observed （P>0.05）.【Conclusion】Compared with the other seed soaking treatments,CeCl₃ treatment had the best effect on improving SOD activity of Allium mongolicum Regel. seeds;generally,the combinative treatments of VC with Ac-DEVD-CHO and CeCl₃ had better effect on removal of ·O₂^- production rate of Allium mongolicum Regel. seeds.

Key words: Allium mongolicum Regel. seeds, Storage year, CeCl₃, VC, Ac-DEVD-CHO, Antioxidant system

中图分类号:

S647

王佳琪, 李娜, 魏鹏超, 杨忠仁, 张东, 张凤兰. Ce³⁺、VC和Ac-DEVD-CHO浸种对沙葱种子抗氧化系统的影响[J]. 北方农业学报, 2021, 49(4): 76-82.

WANG Jiaqi, LI Na, WEI Pengchao, YANG Zhongren, ZHANG Dong, ZHANG Fenglan. Effects of soaking with Ce³⁺,VC and Ac-DEVD-CHO on antioxidant system of Allium mongolicum Regel. seeds[J]. Journal of Northern Agriculture, 2021, 49(4): 76-82.

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Ce³⁺、VC和Ac-DEVD-CHO浸种对沙葱种子抗氧化系统的影响

Effects of soaking with Ce³⁺,VC and Ac-DEVD-CHO on antioxidant system of Allium mongolicum Regel. seeds

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