北方农业学报 ›› 2022, Vol. 50 ›› Issue (6): 10-16.doi: 10.12190/j.issn.2096-1197.2022.06.02

• 作物栽培·生理生化 • 上一篇    下一篇

盐和干旱胁迫对沙葱种子萌发的影响

魏鹏超1, 曹镇宇1, 杨忠仁1,2, 张凤兰1, 韩旭1, 周岩1   

  1. 1.内蒙古农业大学 园艺与植物保护学院/内蒙古自治区野生特有蔬菜种质资源与种质创新重点实验室,内蒙古 呼和浩特 010011
    2.内蒙古自治区农牧业大数据研究与应用重点实验室,内蒙古 呼和浩特 010011
  • 收稿日期:2022-09-20 出版日期:2022-12-20 发布日期:2023-03-24
  • 通讯作者: 杨忠仁
  • 作者简介:杨忠仁(1980—),男,教授,博士,主要从事蔬菜种质资源创新与生理生态等研究工作。
    魏鹏超(1997—),男,硕士研究生,研究方向为沙生蔬菜优势抗逆基因筛选。
  • 基金资助:
    国家自然科学基金项目(31760081);内蒙古自然科学基金项目(2019MS03055);内蒙古自治区种业科技创新重大示范工程揭榜挂帅项目;内蒙古自治区科技重大专项(2019ZD016);内蒙古自治区应用技术研究与开发项目(2019GC237)

Effects of salt and drought stress on seed germination of Allium mongolicum Regel

WEI Pengchao1, CAO Zhenyu1, YANG Zhongren1,2, ZHANG Fenglan1, HAN Xu1, ZHOU Yan1   

  1. 1. College of Horticulture and Plant Protection,Inner Mongolia Agricultural University/Inner Mongolia Key Laboratory of Germplasm Resources and Germplasm Innovation of Wild Specific Vegetables,Hohhot 010011,China
    2. Inner Mongolia Key Laboratory of Research and Application of Agricultural and Animal Husbandry Big Data,Hohhot 010011,China
  • Received:2022-09-20 Online:2022-12-20 Published:2023-03-24
  • Contact: YANG Zhongren

摘要:

【目的】探究盐和干旱胁迫对沙葱种子萌发的影响,为沙葱人工栽培和抗逆性研究提供参考依据。【方法】以沙葱种子为试验材料,采用不同浓度NaCl、 Na2SO4混合的中性盐溶液和PEG-6000溶液分别模拟盐和干旱胁迫,测定其发芽率、发芽势、发芽指数和活力指数,并通过相对发芽率与各处理浓度建立的函数方程确定其种子的耐受性。【结果】随着盐和干旱胁迫的增强,沙葱种子的萌发指标均逐渐下降,初始萌发时间延迟。当盐浓度为75 mmol/L和150 mmol/L时,种子萌发时间分别延迟至第3天和第5天,其发芽率分别下降到23.33%和12.66%;当PEG-6000浓度为15%和20%时,种子萌发时间分别延迟至第3天和第4天,其发芽率分别下降到38.66%和25.33%。沙葱种子耐盐和耐旱半致死渗透势分别为-0.20 MPa和-3.80 MPa,耐盐和耐旱极限渗透势分别为-0.60 MPa和-7.38 MPa。【结论】轻度干旱环境并未明显抑制沙葱种子的萌发,其耐旱极限渗透势为-7.38 MPa;低渗盐溶液显著抑制沙葱种子的萌发,其耐盐极限渗透势为-0.60 MPa。

关键词: 沙葱种子, 盐胁迫, 干旱胁迫, 萌发

Abstract:

【Objective】To explore the effects of salt and drought stress on Allium mongolicum Regel seed germination,and to provide references for the study of artificial cultivation and stress tolerance of Allium mongolicum Regel.【Methods】Taking Allium mongolicum Regel seeds as test material,different concentrations of NaCl and Na2SO4 neutral salt mix solution and PEG-6000 solution were used to simulate salt and drought stress. The germination rate,germination potential,germination index and vitality index were tested. The functional equation created by the relative germination rate and each treatment concentration was used to determine the seed tolerance.【Results】Allium mongolicum Regel seeds germination indicators gradually decreased as salt and drought stress increased,and the initial germination time delayed. When the salt concentration was 75 mmol/L and 150 mmol/L,seeds germination time delayed to the day 3 and day 5,respectively,and the germination rate fell to 23.33% and 12.66%,respectively. When the concentration of PEG-6000 was 15% and 20%,the seeds germination time delayed to day 3 and day 4,respectively,and germination rate fell to 38.66% and 25.33%,respectively. The salt tolerance and drought resistance semi-lethal osmotic potentials were -0.20 MPa and -3.80 MPa,and the salt tolerance and drought resistance limit osmotic potentials were -0.60 MPa and -7.38 MPa,respectively.【Conclusion】The seeds germination of Allium mongolicum Regel was not significantly suppressed under mild drought stress,with drought tolerance limit osmotic potential of -7.38 MPa. Low-permeability salt solution significantly inhibited Allium mongolicum Regel seed germination,with salt tolerance limit osmotic potential of -0.60 MPa.

Key words: Allium mongolicum Regel seeds, Salt stress, Drought stress, Germination

中图分类号: 

  • S633.9