四合木内生放线菌RE4的分离鉴定及其促生活性研究

doi:10.12190/j.issn.2096-1197.2021.05.14

北方农业学报 ›› 2021, Vol. 49 ›› Issue (5): 100-105.doi: 10.12190/j.issn.2096-1197.2021.05.14

四合木内生放线菌RE4的分离鉴定及其促生活性研究

孙莹¹, 甘霖², 林睿¹, 李登高¹, 白薇¹

1.内蒙古农业大学植物逆境生理与分子生物学自治区重点实验室,内蒙古呼和浩特 010018;
2.北京优胜辉煌教育,北京 100000

收稿日期:2021-09-13 发布日期:2022-01-06
通讯作者: 白薇（1972—）,女,教授,博士,主要从事植物抗逆与分子生物学的研究工作。
作者简介:孙莹（1995—）,女,硕士研究生,研究方向为植物抗逆与分子生物学。
基金资助:
国家自然科学基金项目（32060613）

Isolation and identification of endophytic actinomycete RE4 and its growth-promoting activity in Tetraena mongolica

SUN Ying¹, GAN Lin², LIN Rui¹, LI Denggao¹, BAI Wei¹

1. Autonomous Region Key Laboratory of Plant Stress Physiology and Molecular Biology,Inner Mongolia Agricultural University,Hohhot 010018,China;
2. Beijing Outstanding Education,Beijing 100000,China

Received:2021-09-13 Published:2022-01-06

摘要/Abstract

摘要： 【目的】从四合木的根中分离和鉴定内生菌,对其进行抗菌活性筛选,并分析其对四合木种子和植株的促生活性。【方法】利用含有重铬酸钾的高氏一号培养基从四合木根中分离纯化内生菌,并通过生理生化特征和构建 16S rRNA 基因序列系统发育进化树进行菌株鉴定,用对比试验检测该菌株对四合木种子及植株的促生活力,采用平板对峙法检测该菌株的拮抗活性。【结果】从四合木根中分离出的内生菌为放线菌菌株,并命名为RE4。经鉴定,菌株RE4与模式菌株暗灰链霉菌（Streptomyces canus CSSP527^T）的相似性达100%,且进化距离相对模式菌株较近,为链霉菌属（Streptomyces）暗灰链霉菌（Streptomyces canus）;RE4能有效促进四合木种子的萌发及幼苗生长,经RE4处理的四合木幼苗株高、根长、生根率、发芽率比对照分别增加了27.89%、197.09%、26个百分点、4个百分点;RE4可抑制大丽轮枝菌（Verticillium dahliae）、尖孢镰刀菌（Fusarium oxysporum）、核盘菌（Sclerotinia sclerotiorum）和致病疫霉（Phytophthora infestans）的生长,抑菌率分别为68.4%、63.8%、53.8%和38.6%;RE4对酿酒酵母（Saccharomyces cerevisiae）、枯草芽孢杆菌（Bacillus subtilis）、金黄色葡萄球菌（Staphylococcus aureus）、大肠杆菌（Escherichia coli）的抑菌直径分别为17.04、12.45、11.39、11.35 mm。【结论】从四合木根中分离出的内生菌鉴定为链霉菌属（Streptomyces）暗灰链霉菌（Streptomyces canus）,能拮抗多种病原菌,并能促进四合木的种子发芽及幼苗生长。

关键词: 四合木, 内生放线菌, 促生作用, 拮抗活性

Abstract: 【Objective】 Isolation and identification of endophyte from the roots of Tetraena mongolica,screening for antibacterial activity,and analyzing their growth-promoting effects on seeds and plants of Tetraena mongolica. 【Methods】 The endophyte was isolated and purified from Tetraena mongolica roots using Gao's No.1 medium containing potassium dichromate. The strains were identified by physiological and biochemical characteristics and the construction of a phylogenetic tree of 16S rRNA gene sequence. A comparative test was used to detect the growth-promoting activity of the strain on Tetraena mongolica seeds and plants. Plate confrontation method was used to detect the antagonistic activity of the strain. 【Results】 The endophyte isolated from the root of Tetraena mongolica was an actinomycete strain named RE4. After identification,the strain RE4 was 100% similar to the model strain Streptomyces canus CSSP527^T,and the evolutionary distance was relatively close to the model strain,which was Streptomyces Streptomyces canus. RE4 could effectively promote the germination and seedling growth of Tetraena mongolica,the plant height,root length,rooting rate and germination rate of Tetraena mongolica seedlings treated with RE4 increased by 27.89%,197.09%,26 percentage points and 4 percentage points respectively compared with the control. RE4 could inhibit the growth of Verticillium dahliae,Fusarium oxysporum,Sclerotinia sclerotiorum and Phytophthora infestans,and the inhibition rates were 68.4%,63.8 %,53.8% and 38.6%. The inhibitory diameters of RE4 against Saccharomyces cerevisiae,Bacillus subtilis,Staphylococcus aureus and Escherichia coli were 17.04,12.45,11.39,11.35 mm,respectively. 【Conclusion】 The endophyte isolated from the roots of Tetraena mongolica were identified as Streptomyces Streptomyces canus,which could antagonize some pathogens and promote the germination of seeds and the growth of seedlings of Tetraena mongolica.

Key words: Tetraena mongolica, Endophytic actinomycete, Plant growth promotion, Antagonistic activity

中图分类号:

孙莹, 甘霖, 林睿, 李登高, 白薇. 四合木内生放线菌RE4的分离鉴定及其促生活性研究[J]. 北方农业学报, 2021, 49(5): 100-105.

SUN Ying, GAN Lin, LIN Rui, LI Denggao, BAI Wei. Isolation and identification of endophytic actinomycete RE4 and its growth-promoting activity in Tetraena mongolica[J]. Journal of Northern Agriculture, 2021, 49(5): 100-105.

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四合木内生放线菌RE4的分离鉴定及其促生活性研究

Isolation and identification of endophytic actinomycete RE4 and its growth-promoting activity in Tetraena mongolica

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