绿豆晕疫病病原菌的分离和鉴定

doi:10.12190/j.issn.2096-1197.2021.02.13

Abstract

Abstract: 【Objective】To clarify the occurrence of mung bean halo blight and the characteristics of pathogenic bacteria in Inner Mongolia.【Methods】The occurrence of halo blight in mung bean producing areas of 28 banners（counties） in Inner Mongolia from 2014 to 2018 was investigated. The pathogenic bacteria were isolated from the infected mung bean,and the morphology and colony characteristics of the pathogenic bacteria were observed. The pathogenicity,physiological and biochemical characteristics and carbon source utilization were determined,and the pathogenic bacteria were identified combined with molecular biological technology.【Results】The halo blight occurred in all the 28 banners（counties） and the incidence rate was 20%-100%. The strain was identified as Pseudomonas syringae pv. phaseolicola after phenotypic observation,physiological and biochemical identification and molecular biological identification. The utilization degree of carbon source was sucrose>inositol>glucose>lactose.【Conclusion】The halo blight occurred in all the 28 banners（counties） in Inner Mongolia,the pathogenic bacteria was Pseudomonas syringae pv. phaseolicola.

Key words: Mung bean, Halo blight, Pathogen, Identification

CLC Number:

S435.22

TIAN Xiaoyan, KONG Qingquan, HE Xiaoyong, ZHAO Cunhu, CHEN Wenjin, MA Xianmin. Isolation and identification of the pathogen of mung bean halo blight[J].Journal of Northern Agriculture, 2021, 49(2): 84-88.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2021.02.13

https://journal30.magtechjournal.com/bfnyxb/EN/Y2021/V49/I2/84

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Isolation and identification of the pathogen of mung bean halo blight

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