不同百脉根品系茎解剖结构特征分析与抗旱性评价

doi:10.12190/j.issn.2096-1197.2021.04.11

Abstract

Abstract: 【Objective】To screen the strains of Lotus corniculatus with strong drought resistance.【Methods】The stem anatomical structures of four Lotus corniculatus strains（B1,B2,B3 and B4）were observed by paraffin section,the stem cross-sections were analyzed,and eight characteristic parameters of anatomical traits were measured：epidermal cell thickness,cortical cell thickness,xylem thickness,phloem thickness,pulp diameter,stem area,vessel diameter,xylem and phloem area. Variance analysis,principal component analysis,correlation analysis and comprehensive evaluation of drought resistance were carried out.【Results】There was no significant difference in the stem anatomical structure of the four Lotus corniculatus strains. The cross-sectional structure was composed of epidermis,cortex and vascular column. The variance analysis of stem anatomical traits showed that the largest coefficient of variation was the xylem and phloem area,and the smallest was the phloem thickness. The F value of pulp diameter was the largest,while that of phloem thickness was the smallest. Principal component analysis showed that the eigenvalues of the first principal component,the second principal component and the third principal component were 5.089,1.219 and 1.032,respectively,and the cumulative contribution rate reached 91.743% which exceeded 85%,indicating that the first three principal component factors of the four Lotus corniculatus strains could summarize the main information of the eight stem anatomical traits. Correlation analysis showed that the cortical cell thickness was extremely significantly positively correlated with xylem thickness,pulp diameter and stem area（P<0.01）,and the correlation coefficients were 0.970,0.921 and 0.983,respectively. Xylem thickness was extremely significantly positively correlated with pulp diameter and stem area（P<0.01）,and the correlation coefficients were 0.929 and 0.964,respectively. There was a extremely significant positive correlation between pulp diameter and stem area（P<0.01）,and the correlation coefficient was 0.958. The comprehensive analysis of membership function showed that the drought resistance of the four Lotus corniculatus strains was B1>B3>B4>B2.【Conclusion】The eight stem anatomical traits could be used as the anatomical indexes for comparison of drought resistance of Lotus corniculatus strains,and could make more accurate drought resistance identification of Lotus corniculatus;B1 strain had stronger drought resistance.

Key words: Lotus corniculatus, Stem anatomical structure, Drought resistance

CLC Number:

S541.6

LI Hongyan, LI Yuexuan, HU Shun, DUN Huixia. Analysis of stem anatomical structure and evaluation of drought resistance of different strains of Lotus corniculatus[J].Journal of Northern Agriculture, 2021, 49(4): 70-75.

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Analysis of stem anatomical structure and evaluation of drought resistance of different strains of Lotus corniculatus

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