SBPase基因超表达对烟草生长的影响

doi:10.12190/j.issn.2096-1197.2021.04.10

Abstract

Abstract: 【Objective】To investigate the effects of SBPase gene overexpression on agronomic traits and photosynthetic characteristics of tobacco.【Methods】Tobacco leaves were transformed with expression vectors containing SBPase gene to obtain transgenic tobacco lines L1,L2,and L3. Wild-type tobacco WT was used as control,and low-copy transgenic plants were screened by microdrop digital PCR,and agronomic traits and photosynthetic characteristics of transgenic tobacco were measured.【Results】It was found that the stem circumference and leaf length of three transgenic tobacco lines was significantly（P<0.05） higher than that of wild-type,the stem circumference increased by 52.47%,21.34%,and 49.17%,respectively;leaf length increased by 30.77%,25.96%,and 39.10%,respectively. The leaf width was extremely significantly higher（P<0.01）than that of wild-type tobacco by 55.48%,53.82%,and 65.39%,respectively. The number of leaves and plant height of transgenic tobacco were higher than that of wild-type tobacco,but the difference were not significant（P>0.05）. The net photosynthetic rates of three transgenic tobacco lines were significantly（P<0.05）higher than those of wild-type tobacco by 88.2%,43.6%,and 27.1%,respectively. The transpiration rate and stomatal conductance were slightly higher than those of wild-type tobacco,but the differences were not significant（P>0.05）. Intercellular CO₂ concentration was significantly（P<0.05）higher than that of wild-type tobacco,which was 13.0%,4.4%,and 4.3% higher,respectively.【Conclusion】The overexpression of SBPase gene greatly improved the photosynthetic performance of tobacco,which could accumulate more photosynthetic products and promote its own growth.

Key words: Tobacco, SBPase gene, Overexpression, Agronomic traits, Photosynthetic characteristics, Microdrop digital PCR

CLC Number:

S572

HAN Ping′an, SUN Ruifen, TANG Kuangang, CHANG Yue, LIANG Yahui, NIE Lizhen, WU Xinrong, LI Xiaodong. Effects of SBPase gene overexpression on tobacco growth[J].Journal of Northern Agriculture, 2021, 49(4): 62-69.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2021.04.10

https://journal30.magtechjournal.com/bfnyxb/EN/Y2021/V49/I4/62

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Effects of SBPase gene overexpression on tobacco growth

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