不同燕麦品种抗氧化系统对秆锈病菌侵染的响应

doi:10.12190/j.issn.2096-1197.2021.04.14

Abstract

Abstract: 【Objective】To investigate the effects of stem rust infection on antioxidant system of different oat varieties.【Methods】The susceptible varieties Bayou No. 1 and Mengyan No. 1 and the resistant varieties Bayou No.18 and Baiyan No. 5 were selected as the test materials,and distilled water as a control,to analyze the changes of reactive oxygen species content and antioxidant enzyme activities in different organs of oat after inoculation with stem rust bacteria for 1,3,5,7,9 d.【Results】The changes of reactive oxygen species in leaves of susceptible and resistant oat varieties were different after inoculation. H₂O₂ content in leaves of resistant oat varieties was more accumulated than that of susceptible oat varieties,and the increase of ·O₂^- content in susceptible oat varieties was greater than that of resistant oat varieties. The activities of SOD,POD,PAL and CAT in oat leaves after inoculation with stem rust bacteria were higher than those in CK. After inoculation,SOD activity in leaves of susceptible and resistant oat varieties reached peak values on day 3 and 9,respectively. Compared with CK,susceptible variety Mengyan No. 1 was significantly increased by 63.2% and 47.5%（P<0.05）,and Bayou No. 1 was significantly increased by 50.0% and 73.8%（P<0.05）,respectively. During 7 to 9 days after inoculation,CAT activity in leaves of Bayou No. 1 and Mengyan No. 1 was significantly increased by 24.8%-30.7% and 20.4%-30.0% compared with CK（P<0.05）,respectively.During 5 to 9 days after inoculation,CAT activity in leaves of Bayou No. 18 and Baiyan No. 5 was significantly increased by 27.6%-55.8% and 37.2%-48.3%（P<0.05）,respectively.【Conclusion】After inoculation with stem rust bacteria,resistant oat varieties produced reactive oxygen species quickly and increased the activity of defense enzymes,which resulted in earlier defense response and improved disease resistance than susceptible varieties.

Key words: Oat, Stem rust bacteria, Reactive oxygen species, Defense enzymes

CLC Number:

S513

HAN Yuting, CHEN Xiaojing, XU Zhongshan, LIU Jinghui, XUE Guoxing. The response of antioxidant system of different oat varieties to infection of stem rust[J].Journal of Northern Agriculture, 2021, 49(4): 98-104.

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The response of antioxidant system of different oat varieties to infection of stem rust

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