Bacillus megaterium L6 菌株防治燕麦炭疽病的研究

doi:10.12190/j.issn.2096-1197.2023.03.07

Abstract

Abstract: 【Objective】To screen biocontrol bacteria having control efficacy against oat anthracnose.【Methods】Using plate antagonistic test and growth promoting pot test,four treatments were set up including,spraying clear water（CK）,spraying anthrax spore suspension,spraying anthrax spore suspension plus Bacillus megaterium L6 suspension,and spraying Bacillus megaterium L6 suspension. The activity of Phenylalanine ammoniase lyase（PAL） and the content of Malondialdehyde（MDA）were measured and calculated by UV-VIS spectrophotometer.【Results】L6 strain and Bacillus megaterium OUCMDZ4181 were grouped together by phylogenetic analysis,and were identified as Bacillus megaterium. Plate antagonism test showed that the inhibition rate of Bacillus megaterium L6 strain to anthrax was 89.89%. After anthrax spore suspension plus Bacillus megaterium L6 suspension treatment,the content of MDA in 9 d decreased to 28.07% of that of anthrax spore suspension treatment,whereas the activity level of PAL increased compared with anthrax spore suspension treatment. Both inoculation of Bacillus megaterium L6 strain alone and combined inoculation with anthrax had growth promoting effect on oat plants,with the growth promoting rates of seedling height and root length of 15.13%,25.40% and 11.06%,17.24%,respectively.【Conclusion】The Bacillus megaterium L6 strain could stimulate the induced resistance of oat to anthrax and promote the growth of oat root and seedling.

Key words: Oat, Anthracnose, Endophytic bacteria, Induced resistance

CLC Number:

S435.126

LI Jiale, KONG Haoran, HE Xiaoshi, GUO Yuhang, NIU Jingheng, DONG Baozhu, BAO Haizhu, HAN Shengcai. Study on the control of oat anthracnose by Bacillus megaterium L6[J].Journal of Northern Agriculture, 2023, 51(3): 59-65.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2023.03.07

https://journal30.magtechjournal.com/bfnyxb/EN/Y2023/V51/I3/59

References

[1] 何苏琴,荆卓琼,赵桂琴,等.内蒙古自治区乌兰察布市燕麦炭疽病病原鉴定[J].植物保护,2015,41(3):68-72.
[2] 黄保全,黄丽丽,康振生,等.小麦内生枯草芽孢杆菌E1R-j胞外抗菌蛋白的分离纯化和性质[J].西北农业学报,2009,18(6):285-290.
[3] 孙浩洋,张炜炜,曾亮,等.不同生防药剂对燕麦白粉病的防治效果[J].草业科学,2019,36(9):2374-2383.
[4] 徐亚英,黄晓梅,谢丽.枯草芽孢杆菌液体发酵条件优化探究[J] .南方农业,2020,14(20):139-142.
[5] 何艺琴,卢晨,徐文杰,等.小麦内生细菌XG-7对赤霉病菌的拮抗作用及其生物学特性[J].甘肃农业大学学报,2019,54(1):110-116.
[6] 刘继霞,山军建,王平.两种生防菌剂防治向日葵黄萎病的效果[J].中国植保导刊,2020,40(12):80-81.
[7] YANG F Z,ZHANG R Y,WU X Y,et al.An endophytic strain of the genus Bacillus isolated from the seeds of maize(Zea mays L.) has antagonistic activity against maize pathogenic strains[J].Microbial Pathogenesis,2020,142:104074.
[8] 姬婧媛,杨洁,高小宁,等.植物内生枯草芽孢杆菌E1R-j脂肽类化合物的分离鉴定及抑菌作用[J].农药学学报,2015,17(2):172-178.
[9] 牛焕杰,李辉,王娜娜,等.苹果树腐烂病菌拮抗枯草芽孢杆菌E1R-j抗菌蛋白的分离纯化[J].西北农林科技大学学报(自然科学版),2016,44(9):135-142.
[10] 李晴,柴霜,冯千禧,等.植物内生菌在农作物方面的应用探析[J].种子科技,2021,39(12):8-9.
[11] 梁振霆,唐婷.内生菌对植物次生代谢产物的生物合成影响和抗逆功能研究[J].生物技术通报,2021,37(8):35-45.
[12] MARAG P S,SUMAN A.Growth stage and tissue specific colonization of endophytic bacteria having plant growth promoting traits in hybrid and composite maize(Zea mays L.)[J].Microbiological Research,2018,214:101-113.
[13] 王苗苗,周向睿,梁国玲,等.5份燕麦材料苗期耐盐性综合评价[J].草业学报,2020,29(8):143-154.
[14] 王海霞,郑成忠,东保柱,等.燕麦内生细菌YN-J3的分离鉴定及防病促生作用研究[J].中国生物防治学报,2022,38(2):447-457.
[15] 张志良,瞿伟菁,李小方.植物生理学实验指导[M].4版.北京:高等教育出版社,2009.
[16] 刘彦策,王会敏,钱欣雨,等.玉米内生菌L10的分离、鉴定及拮抗活性[J].植物保护学报,2021,48(3):630-637.
[17] 畅涛,杨成德,薛莉,等.珠芽蓼内生菌ZA1对马铃薯的防病促生研究[J].草业学报,2015,24(12):83-91.
[18] 刘丽. 小麦内生菌的分离与鉴定[J].沈阳师范学院学报(自然科学版),1998,16(4):41-46.
[19] 李岚岚,戴利铭,蒋桂芝,等.橡胶树炭疽病生防内生菌的分离鉴定及抑菌作用研究[J].热带作物学报,2021,42(10):2958-2965.
[20] 陆娟,唐娟,王贵生,等.亳菊内生菌BN7的鉴定与生防促生功能研究[J].天然产物研究与开发,2021,33(8):1301-1307.
[21] 刘惠英,朱志强,陈嘉敏,等.凤梨内生菌筛选鉴定及拮抗效果评价[J].湖北大学学报(自然科学版),2020,42(2):158-164.
[22] 丁从文,韦罗晴,马忠璇,等.巨大芽孢杆菌LB01粗提物对采后芒果炭疽病的生防效果及成分鉴定[J].食品研究与开发,2021,42(17):31-36.
[23] 廖庭,秦健,袁高庆,等.巨大芽孢杆菌B196菌株抑菌物质的分离纯化[J].植物保护,2014,40(2):16-21.
[24] 周东兴,王恩泽,刘多,等.番茄枯萎病生防细菌的筛选及对植株防御酶活性的影响[J].生态学杂志,2020,39(5):1753-1760.
[25] 周园园,郭永霞,段玉玺,等.巨大芽孢杆菌Sneb207诱导大豆抗胞囊线虫病的防效及光合响应[J].大豆科学,2020,39(4):605-611.
[26] 黄姗姗,段玉玺,陈立杰,等.诱导大豆抗逆细菌的筛选及分子鉴定[J].大豆科学,2011,30(2):205-210.
[27] 刘江苇,刘颖,徐婷,等.水稻内生菌研究进展及展望[J].生命科学研究,2021,25(3):232-239.
[28] 刘亚东,刘景辉,卿香玉,等.生物菌肥对燕麦叶片生理特性及产量的影响[J].北方农业学报,2019,47(3):58-63.

Study on the control of oat anthracnose by Bacillus megaterium L6

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	ZHENG Chengzhong, XU Zhenpeng, ZHANG Zizhen, WANG Qianjun, MEI Xue, SUN Ying, WANG Fengwu, YE Lu. Comprehensive evaluation of agronomic,quality traits and adaptability of new naked oat varieties （lines） [J]. Journal of Northern Agriculture, 2023, 51(2): 12-21.
[2]	SUN Yueying, LIU Jinghui, MI Junzhen, ZHAO Baoping, LI Yinghao. The effect of lactobacillus compound preparation on the formation of oat population structure and root growth [J]. Journal of Northern Agriculture, 2023, 51(2): 33-40.
[3]	WANG Yaping, XU Linbo, WANG Lan, LIN Kejian, CUI Jin, ZHANG Quanyi. Identification of aphid resistance of different oat varieties and comprehensive evaluation of the production performance [J]. Journal of Northern Agriculture, 2022, 50(6): 72-79.
[4]	ZHANG Lu, ZHAI Xiaoyu, WU Junying, GAO Shihua, WANG Xuefeng, ZHAO Yufei. Evaluation on the regeneration capacity of different oat varieties after cutting [J]. Journal of Northern Agriculture, 2022, 50(4): 26-34.
[5]	FU Jia, LIU Tingting, ZHANG Hui, JIANG Shanqiang, ZHOU Li, MA Dan, TIAN Xuejun, ZHU Deyan, XU Yan. Study on the mechanism of salicylic acid inducing rice blast resistance [J]. Journal of Northern Agriculture, 2022, 50(4): 47-54.
[6]	BAI Jianhui, WANG Yuqing. Effects of exogenous spermine on photosynthetic characteristics of oat under alkali stress [J]. Journal of Northern Agriculture, 2022, 50(3): 9-16.
[7]	TAO Bo, ZHAO Xu, DU Lei, YUN Xiaopeng. Study on induction of sunflower resistance by elicitor [J]. Journal of Northern Agriculture, 2022, 50(3): 53-59.
[8]	ZHANG Zhifen, FU Xiaofeng, YANG Haishun, FENG Baili, GAO Jinfeng, CUI Siyu, CHAI Yan, LIU Junqing. Evaluation of high yield and adaptability of naked oats（lines） [J]. Journal of Northern Agriculture, 2021, 49(5): 1-8.
[9]	HAN Yuting, CHEN Xiaojing, XU Zhongshan, LIU Jinghui, XUE Guoxing. The response of antioxidant system of different oat varieties to infection of stem rust [J]. Journal of Northern Agriculture, 2021, 49(4): 98-104.
[10]	YU Zhiguang, YAN Jie, MI Junzhen, ZHAO Baoping, LYU Pin, ZHANG Ru, WU Junying, LIU Jinghui. Effects of water and drought conditions on photosynthesis characteristics and assimilate accumulation on different oat varieties [J]. Journal of Northern Agriculture, 2021, 49(3): 22-28.
[11]	ZHANG Jun, DUAN Yu, LIU Yahong, LIANG Junmei, WANG Bo, AN Hao, LIAN Haifei. Effects of different fertilization treatments on oat yield,nutrient absorption and water and fertilizer utilization efficiency [J]. Journal of Northern Agriculture, 2020, 48(5): 62-68.
[12]	WANG Lu, WANG Fengwu, GAO Qing, LIU Jinghui, ZHAO Baoping. Effect of different sowing date and combined application of nitrogen and phosphorus fertilizers on oats yield [J]. Journal of Northern Agriculture, 2020, 48(3): 80-85.
[13]	ZHANG Zhifen, FU Xiaofeng, LIU Junqing, YANG Haishun, HE Jiangfeng, WANG Liwei. Effect of differet drought stress treatment on yield and leaves ultrastructure of oat [J]. Journal of Northern Agriculture, 2020, 48(1): 41-45.
[14]	ZHANG Zhifen, FU Xiaofeng, LIU Junqing, YANG Haishun. Breeding and agronomic character analysis of the new shelled oat variety Mengyan 3 [J]. Journal of Northern Agriculture, 2019, 47(6): 28-33.
[15]	SUN Yannan, QI Bingjie, HE Xin, WANG Min, LIU Jinghui. Effect of different supplying levels of Zn on the growth and Zn accumulation of different varieties of oats [J]. Journal of Northern Agriculture, 2019, 47(4): 15-21.