Abstract: 【Objective】To determine the optimal temperature and time for dry-heat treatment to destroy fungi and bacteria mixed in cucumber seed without affecting seed germination.【Methods】Cucumber seeds were dried at 60,70,80,90,and 100 ℃ for 2,4,6,8,10,and 12 h,with seeds without dry-heat treatment used as control（CK）. The germination energy and germination rate were assessed. The inactivation rates of fungal and bacterial on cucumber seed were tested in PDA and LB medium.【Results】The germination rate of cucumber seeds decreased significantly（P<0.05）when the dry-heat treatment was over 80 ℃. In PDA medium,the inactivation rate of fungi was significantly higher than that of CK（P<0.05）after treatment at 70 ℃ and 80 ℃ for 2,4,6,8,10,and 12 h,with the inactivation rate of 70 ℃ for 4 h being the highest,at 97.33%. In LB medium,after treatment at 70 ℃ and 80 ℃ for 2,4,6,8,10,and 12 h,the inactivation rate of bacteria was significantly higher than that of CK（P<0.05）,with the inactivation rate of 70 ℃ for 4 h being the highest,at 96.67%.【Conclusion】Under dry-heat treatment,cucumber seeds germinated normally and the optimal treatment of best inactivation efficacy of fungi and bacteria was 70 ℃ for 4 h.

Key words: Cucumber seeds, Germination rate, Dry-heat treatment, Inactivation rate