山丹种子表面灭菌方法比较试验

doi:10.12190/j.issn.2096-1197.2022.01.12

Abstract

Abstract: 【Objective】Select the optimal seed surface sterilization method to improve Lilium pumilum′s aseptic seeding procedure.【Methods】Lilium pumilum seeds were cultured using aseptic sowing method. Seeds were disinfected with 1.0% NaClO or 0.1% HgCl₂ with gradient treatment duration（0-30 min）. Seeds were then cultured on MS medium mixed with 6-BA 1.0 mg/L and NAA 0.1 mg/L. The germination and growth were monitored regularly.【Results】The contamination rate of 0.1% HgCl₂ treatment was lower than that of 1.0% NaClO treatment at the same treatment time. Lilium pumilum seeds germination and growth were better with 0.1% HgCl₂ treatment than with 1.0% NaClO treatment. Seed germination and growth increased initially then declined as treatment duration was extended. The best treatment was 15 min of 0.1% HgCl₂,which had a 97.78% germination rate and superior seedling growth than the other treatments.【Conclusion】Lilium pumilum seeds had higher germination rate and better seedling growth after being treated with 0.1% HgCl₂ for 15 min.

Key words: Lilium pumilum seeds, Tissue culture, Surface sterilization, Contamination rate, Germination rate, Induction rate

CLC Number:

S682.29

DENG Yu, WANG Meng, LI Fahu, LI Longmei, HOU Jia. Comparative study on surface sterilization methods for Lilium pumilum seed[J].Journal of Northern Agriculture, 2022, 50(1): 87-92.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2022.01.12

https://journal30.magtechjournal.com/bfnyxb/EN/Y2022/V50/I1/87

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Comparative study on surface sterilization methods for Lilium pumilum seed

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