Abstract:

【Objective】Screening transcription factors related to soybean protein synthesis and forecasting potential genes and metabolic pathways for protein synthesis.【Methods】Using RNA-Seq technology,high-throughput sequencing of soybean seeds was carried out on Zhongdou 4901,a low protein soybean material,and Huaidou 98-28,a high protein soybean material.【Results】43.14 Gb Clean Data were obtained. Each sample had a data volume greater than 6.46 Gb,a GC content greater than 45.73%,a Q20 base percentage greater than 94.78%,and a Q30 base percentage greater than 88.53%. The data quality complied with the standards. 916 genes that were differentially expressed,248 of which were up-regulated and 668 were down-regulated. The screened differentially expressed transcription factors came from 26 transcription factor families,of which 4 families,b HLH,AP2/ERF,MYB,and B3,may contribute to soybean protein synthesis.【Conclusion】The Sec61 gene of protein conduction channel in the endoplasmic reticulum processing pathway of soybean protein was down regulated,resulting in the decrease of protein synthesis ability and decrease of protein content in some varieties. The Sec61 gene was thought to be the crucial gene for protein synthesis. Protein endoplasmic reticulum processing,thiometabolism,and glutathione metabolism were related metabolic mechanisms that contribute to the difference in protein content between varieties.

Key words: Soybean, Protein content, RNA-Seq