Abstract:

【Objective】To clarify the effects of different microbial inoculants on biomass of continuous cropping A. membranaceus var. mongholicus and soil fungal community structure.【Methods】 Four treatments were set up using A. membranaceus var. mongholicus as test material，ETS compound inoculant（ETS），Doveite antibacterial inoculant（DWTK），multifunctional vitamin bacterial inoculant（WSS）and blank control（CK）. The biomass of A. membranaceus var. mongholicus was measured. And high-throughput sequencing technology was used to examine the fungal community structure in the soil.【Results】The biomass of A. membranaceus var. mongholicus above ground tissues and roots was significantly（P<0.05）increased by DWTK treatment，with above ground biomass increased by 17.5% and root biomass increased by 18.4%，compared with CK. The three microbial inoculants clearly improved the soil fungal community. Among them，the number of soil fungi OTU in DWTK treatment was substantially higher than that of CK and WSS treatment，and the fungal groups were more abundant. In comparison to the other three treatments，the DWTK treatment significantly reduced the abundance of pathogenic fungi Fusarium and increased the abundance of Gibberella.【Conclusion】Doveite antibacterial inoculant effectively improved the structure of rhizosphere fungi community of A. membranaceus var. mongholicus，reduced the number of pathogenic fungi，and increased the number of fungi. It was advantageous to strengthen the continuous cropping A. membranaceus var. mongholicus disease resistance and boost the biomass.

Key words: Astragalus membranaceus var. mongholicus, Biomass, Microbial inoculants, Fungi community structure