平安系列小麦品种（系）穗发芽抗性鉴定及基因的分子标记检测

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Identification of pre-harvest sprouting resistance and molecular marker detection of genes in Ping′an series wheat varieties（lines）

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doi:10.12190/j.issn.2096-1197.2024.06.02

Abstract: 【Objective】To investigate the pre-harvest sprouting resistance and genetic composition of Ping′an series wheat varieties（lines）.【Methods】The pre-harvest sprouting characteristics of 14 Ping′an series varieties（lines） were analyzed by whole ear germination method,and the sources of pre-harvest sprouting resistance genes were detected by molecular markers of 5 anti pre-harvest sprouting genes.【Results】Among the 14 varieties（lines）,the relative pre-harvest sprouting index of Ping′an No. 11 was 0.19,reached the level of resistance;the relative pre-harvest sprouting indexes of Ping′an 658 and Ping′an 701 were 0.35 and 0.37,respectively,reached the level of moderately resistance. The results of molecular marker detection of anti pre-harvest sprouting genes showed that the relative pre-harvest sprouting index of haplotype composed of three loci （-222、+646、+666） of TaPHS1 gene was CGAC<TGAC<TGAT<TATT from low to high,and Ping′an No. 11 contained the best haplotype of TaPHS1 gene of anti pre-harvest sprouting,7 wheat varieties（lines） including Ping′an 901 contained anti pre-harvest sprouting gene TaVp1B3,4 wheat varieties including Ping′an 12 contain anti pre-harvest sprouting gene PM19A-1,all 14 wheat varieties（lines） contain neither of anti pre-harvest sprouting gene Dorm-1 or Tamyb10-D.【Conclusion】Ping′an No. 11 is the only variety with hap-CGAC resistant to pre-harvest sprouting,which can be used in cultivating new varieties of anti pre-harvest sprouting wheat and discovering new anti pre-harvest sprouting gene.

Key words: Wheat, Pre-harvest sprouting resistance, Evaluation of resistance, Germination index, Molecular marker, Allelic variation

CLC Number:

S512.1

LYU Qingfeng, ZHU Changtao, WANG Gang, LYU Yuanfeng, YAN Zedong, ZHANG Zhen, ZHANG Lilin. Identification of pre-harvest sprouting resistance and molecular marker detection of genes in Ping′an series wheat varieties（lines）[J].Journal of Northern Agriculture, 2024, 52(6): 13-21.

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