Abstract: 【Objective】 To study the color wheat tissue culture regeneration system and provide a theoretical basis for establishing a high-frequency color wheat genetic transformation system. 【Methods】 The mature embryos of three color wheat varieties（lines）of Zhongheimai No.6,CM-Z2 and Zhonglanmai No.1 were used. The induction status was recorded at 8,14,and 20 days after callus induction. Growth regulator IAA+KT and IAA+6-BA combination were used to redifferentiate color wheat callus. The callus rate,differentiation rate and seedling rate of different treatments were evaluated. 【Results】 The explants of Zhongheimai No.6,CM-Z2 and Zhonglanmai No.1 had the best callus induction effect after cultured for 14 days,with callus rates ranging from 80.54% to 95.39%. The regeneration ability of color wheat mature embryo callus was related to genotype and culture medium. Zhongheimai No.6 had the best regeneration ability,with a differentiation rate of 87.68%-97.50%,and a seedling rate of 17.26%-61.73%. The seedling rate was higher in the differentiation medium containing 0.1 mg/L IAA and 0.5 mg/L 6-BA,0.5 mg/L IAA and 4 mg/L KT,with a rate of 61.73% and 56.25% respectively. CM-Z2 followed Zhongheimai No.6,had a differentiation rate of 60.23%-95.45% and seedling rate of 2.27%-34.09%. CM-Z2 had the highest seedling rate in the differentiation medium containing 0.5 mg/L IAA and 4 mg/L KT,reaching 34.09%. 【Conclusion】 Zhongheimai No.6 was an ideal material for color wheat genetic transformation. Regeneration medium containing 0.1 mg/L IAA and 0.5 mg/L 6-BA,0.5 mg/L IAA and 4 mg/L KT were suitable for Zhongheimai No.6 and CM-Z2.

Key words: Color wheat, Tissue culture, Mature embryo, Regeneration system