基于SSR分子标记的早熟棉种质资源遗传多样性分析

doi:10.12190/j.issn.2096-1197.2022.01.02

Abstract

Abstract: 【Objective】 To clarify the genetic relationship of the early maturing cotton germplasm resources in the Cotton Research Center of the Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,in order to provide parental materials for hybrid combination selection and directional breeding.【Methods】 Using SSR molecular markers（microsatellite DNA）,31 pairs of SSR primers were used to investigate and identify the genetic diversity of 44 early maturing cotton germplasm resources.【Results】 31 pairs of primers amplified a total of 72 polymorphic bands,with an average of 2.25 bands per pair of primers. The genetic similarity coefficient among the materials ranged from 0.35 to 0.80,with an average of 0.58,according to the results of cluster analysis. The genetic similarity coefficient between Jiumian No.17 and Zhong 716 was the lowest,at 0.35. The highest genetic similarity coefficient were 0.80 for Xinluzao No.41 and Zhong 361,Zhongxia 05 and Liaomian No.15.【Conclusion】The 31 pairs of SSR primers were effective in amplifying the polymorphisms among the materials studied,and the genetic diversity types of 44 early maturing cotton materials were abundant,revealed the genetic background differences and genetic relationship among early maturing cotton germplasm resources.

Key words: Early maturing cotton, Germplasm resources, Genetic diversity, SSR molecular markers

CLC Number:

S562

XIAN Feng, LU Zhanyuan, ZHANG Jianzhong, CHEN Liyu, CHENG Yuchen, YANG Jianqiang, ZHANG Xiangqian, Suhe. Genetic diversity analysis of early maturing cotton germplasm resources base on SSR molecular markers[J].Journal of Northern Agriculture, 2022, 50(1): 11-17.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2022.01.02

https://journal30.magtechjournal.com/bfnyxb/EN/Y2022/V50/I1/11

References

[1] 张华峰. 中国棉花产业国际竞争力及影响因素分析[D].泰安:山东农业大学,2016.
[2] 滕中华,王晓雯,张建,等.棉花栽培品种/品系的SSR标记遗传多样性分析[J].西南大学学报(自然科学版),2012,34(12):20-26.
[3] 匡猛,杨伟华,许红霞,等.中国棉花主栽品种DNA指纹图谱构建及SSR标记遗传多样性分析[J].中国农业科学,2011,44(1):20-27.
[4] 贾子昉,赵海红,李成奇,等.棉花种质资源遗传多样性研究进展[J].贵州农业科学,2014,42(1):16-20.
[5] 张国裕,张帆,姜立纲,等.南瓜属EST-SSR标记的开发及在杂种纯度鉴定中的应用[J].华北农学报,2011,26(6):97-101.
[6] REN Y,WU J,ZHAO J,et al.Identification of SSR markers closely linked to the yellow seed coat color gene in heading Chinese cabbage(Brassica rapa L. ssp.pekinensis)[J].Biology Open,2017,6(2):278-282.
[7] KAPOOR M,MAWAL P,SHARMA V,et al.Analysis of genetic diversity and population structure in Asparagus species using SSR markers[J].Journal Genetic Engineering & Biotechnology,2020,18(1):50.
[8] PREETHI P,RAHMAN S,NAGANEESWARAN S,et al.Development of EST-SSR markers for genetic diversity analysis in coconut(Cocos nucifera L.)[J].Molecular Biology Reports,2020,47(12):9385-9397.
[9] BOURGOU L,SAWADOGO M,SANFO D,et al.SSR-based genetic diversity of traditional and perennial cotton(Gossypium spp.)populations collected in Burkina Faso[J].Genetic Resources and Crop Evolution,2017,64(7):1743-1759.
[10] 贾子昉,王清连,董娜.陆地棉种质资源的表型及SSR遗传多样性分析[J].生物技术通讯,2019,30(5):653-661.
[11] 石建斌,周红,王宁,等.棉花SSR标记种质资源纯度鉴定及遗传多样性分析[J].生物技术通报,2018,34(7):138-146.
[12] 卫泽,孙学振,柳宾,等.国内外57份棉花种质资源的遗传多样性研究[J].山东农业科学,2010(6):13-18.
[13] 高伟,刘方,黎绍惠,等.利用SSR分析四倍体棉种多态性[J].作物学报,2010,36(11):1902-1909.
[14] 张大伟,李海燕,张道远,等.适用于PCR的棉花基因组DNA快速、批量制备方法的探讨[J].中国棉花,2014,41(8):10-14.
[15] 张玉翠,杨伟华,匡猛,等.32个棉花主栽品种DNA指纹图谱构建及遗传多样性分析[J].棉花学报,2012,24(2):120-126.
[16] 潘兆娥,孙君灵,王西文,等.基于棉花参比种质的SSR多态性核心引物筛选[J].生物多样性,2008,16(6):555-561.
[17] 贺道华,邢宏宜,李婷婷,等.92份棉花资源遗传多样性的SSR分析[J].西北植物学报,2010,30(8):1557-1564.
[18] 董章辉,赵丽芬,赵彦坤,等.83份早熟抗虫棉种质资源的SSR标记聚类分析[J].华北农学报,2015,30(S1):46-50.
[19] 陈浩东,肖才升,李庠,等.基于SSR标记的短季棉品种DNA指纹图谱构建及遗传多样性分析[J].华北农学报,2014,29(4):98-104.
[20] 王欣怡,李雪源,龚照龙,等.基于SSR标记新疆陆地棉的DNA指纹图谱构建及遗传多样性分析[J].棉花学报,2018,30(4):308-315.
[21] 翟书伟,邓婷婷,曹云泉,等.基于SSR标记的26份棉花材料的遗传多样性分析[J].种子,2020,39(10):67-72.
[22] MALIK W,ASHRAF J,IQBAL M Z,et al.Molecular markers and cotton genetic improvement:current status and future prospects[J].The Scientific World Journal,2014,2014:607091.
[23] YANG Z,QANMBER G,WANG Z,et al.Gossypium genomics:trends,scope,and utilization for cotton improvement[J].Trends in Plant Science,2020,25(5):488-500.

Genetic diversity analysis of early maturing cotton germplasm resources base on SSR molecular markers

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	GUO Chengyu, YI Fengyan, FANG Yongyu, SHI Zhidan, LIU Fang, ZHAO Heping, DING Haijun. Genetic diversity analysis of phenotypic traits in 99 barley germplasm resources [J]. Journal of Northern Agriculture, 2023, 51(4): 1-10.
[2]	ZHANG Qi, SUN Rujian, GUO Rongqi, YU Ping, FENG Lei, CHAI Shen, HU Xingguo, SUN Bincheng. Research progress and utilization status of dwarf and density tolerant soybean germplasm resources [J]. Journal of Northern Agriculture, 2022, 50(6): 25-30.
[3]	SUN Pingping, LI Zhengnan, LIU Xin, ZHANG Lei, WANG Yong, FU Chongyi. Resistance evaluation of pepper germplasm resources against pepper Phytophthora and fungicide sensitivity analysis on Phytophthora capsici [J]. Journal of Northern Agriculture, 2022, 50(5): 67-72.
[4]	YANG Fan, ZHANG Yanfang, WANG Jinhua, ZHAO Yuan, ZHAO Lingmin, ZHANG Xiaomeng, ZHANG Yong, HUO Xiuwen. SSR analysis of Chinese yam（Dioscorea opposita Thunb.）germplasm resources and construction of primary core collection [J]. Journal of Northern Agriculture, 2022, 50(2): 18-27.
[5]	HAN Zhigang, XIE Rui, JIN Xiaolei, GUO Binyu, GUO Jingshan. Genetic diversity analysis of potato germplasm resources based on phenotypic traits [J]. Journal of Northern Agriculture, 2021, 49(5): 9-17.
[6]	GUO Jiang′an, FENG Yong, ZHAO Ruixia, HAN Yuting, SU Erhu, LIANG Hongwei, ZHAO Yiwen, ZHANG Laihou. Analysis of genetic diversity and identification of stem rot resistance in maize backbone inbred lines [J]. Journal of Northern Agriculture, 2021, 49(4): 30-34.
[7]	ZHAO Shangmin, LI Xiaodong, FU Zengjuan, E Yuanyuan, ZHANG Hui, WANG Liang, ZHANG Ziqiang, ZHENG Wenzhe, ZHANG Bizhou, BAI Chen, ZHANG Huizhong. Analysis of genetic diversity and population structure of sugarbeet germplasm resources by SSR markers [J]. Journal of Northern Agriculture, 2021, 49(1): 1-9.
[8]	CUI Guohui, YE Jun, WU Xiaohua, YANG Lei, WANG Xiaobing, TAN Liping, YU Meiling, ZHAO Chunzhi, ZHANG Chunyan, ZHANG Haibin, WU Yunxia, LI Yuanqing. Genetic diversity analysis of phenotype traits of spring wheat parental resources in Inner Mongolia [J]. Journal of Northern Agriculture, 2020, 48(3): 1-9.
[9]	CHEN Wenjin, ZHAO Cunhu, HE Xiaoyong, KONG Qingquan, ZHANG Ziqiang, CHEN Wenhe, YANG Jie. Genetic diversity analysis of agronomic characters of mung bean main cultivars [J]. Journal of Northern Agriculture, 2020, 48(3): 18-25.
[10]	PANG Peng, ZHANG Zhiyong, LI Lijun, QI Bingjie, GUO Zhanbin. Genetic diversity analysis of agronomic characters of quinoa germplasm resources [J]. Journal of Northern Agriculture, 2020, 48(3): 26-31.
[11]	YE Jun, WU Xiaohua, LI Yuanqing, CUI Guohui, WANG Xiaobin, ZHAO Chunzhi, YANG Lei, ZHANG Sanfen, ZHANG Haibin, YU Meiling. Genetic diversity analysis of phenotypic traits in quinoa germplasm resources [J]. Journal of Northern Agriculture, 2020, 48(1): 1-6.
[12]	ZHANG Rui, TANG Aijia, XIE Jihong, SHI Fengling, ZHAO Yan. DNA fingerprinting and genetic diversity analysis of Elymus pastures [J]. Journal of Northern Agriculture, 2019, 47(5): 1-8.
[13]	WANG Shanshan, ZHOU Changyan, ZHANG Xiangqian, LU Zhanyuan, YAN Jingbin, HAO Jianxi, CHENG Yuchen, ZHANG Chunyan. Yield difference analysis and genetic diversity of the main agronomic traits in aerospace breeding wheat strains [J]. Journal of Northern Agriculture, 2019, 47(3): 1-6.
[14]	CHEN Wenjin, KONG Qingquan, ZHAO Cunhu, HE Xiaoyong ,TIAN Xiaoyan, ZHANG Xiangqian, XI Xianmei （Plant Protection Institute,Inner Mongolia Academy of Agricuhural ＆ Animal Husbandry Sciences,Hohhot 010031, China）. Analysis of genetic diversity of the main agronomic traits of chickpea germplasm resources [J]. Journal of Northern Agriculture, 2018, 46(5): 9-18.
[15]	. Application of DNA molecular markers in Pleurotus eryngii [J]. Journal of Northern Agriculture, 2017, 45(6): 28-28.