枯草芽孢杆菌Tpb55和棘孢木霉HG1复合菌水分散粒剂的研制与应用

doi:10.12190/j.issn.2096-1197.2025.04.09

Abstract

Abstract: 【Objective】To investigate the control efficacy of Bacillus subtilis Tpb55 and Trichoderma asperellum HG1 compound bacteria water dispersible granules on tobacco black shank and tobacco brown spot. 【Methods】Single-factor experiments were conducted to screen and optimize the adjuvants for B. subtilis Tpb55 and T. asperellum HG1 compound bacteria water dispersible granules. Pot and field experiments were carried out to evaluate the control efficacy of the compound bacteria water dispersible granules at 400-fold,800-fold,and 1 200-fold dilutions on tobacco black shank and tobacco brown spot,respectively.【Results】By optimizing the dosage of adjuvants,the optimal formula of the compound bacteria water dispersible granules was determined as follows：5% wetting agent（saponin powder）,6% dispersant（carboxymethylcellulose sodium）,3% binder（polyethylene glycol 6000）,5% disintegrant（sodium carboxymethyl starch）,with diatomaceous earth as the carrier to make up 100%. The 400-fold dilution of B. subtilis Tpb55 and T. asperellum HG1 compound bacteria water dispersible granules exhibited the highest efficacy,achieving an indoor control efficacy of 81.53% on tobacco black shank and a field control efficacy of 70.93% on tobacco brown spot.【Conclusion】The optimal formula of B. subtilis Tpb55 and T. asperellum HG1 compound bacteria water dispersible granules are confirmed,and the 400-fold dilution of compound bacteria water dispersible granules exhibits excellent biocontrol efficacy on tobacco black shank and tobacco brown spot.

Key words: Bacillus subtilis, Trichoderma asperellum, Compound bacteria water dispersible granules, Tobacco black shank, Tobacco brown spot, Control efficacy

CLC Number:

S572

HAN Xiaobin, WANG Xianbo, LUO Yuying, LU Huijun, PENG Zhixin, WEN Mingxia. Preparation and application of Bacillus subtilis Tpb55 and Trichoderma asperellum HG1 compound bacteria water dispersible granules[J].Journal of Northern Agriculture, 2025, 53(4): 99-109.

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Preparation and application of Bacillus subtilis Tpb55 and Trichoderma asperellum HG1 compound bacteria water dispersible granules

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