黄花苜蓿Mfβ-Hex1基因的克隆及生物信息学分析

doi:10.3969/j.issn.2096-1197.2019.03.10

Abstract

Abstract: In order to identify the grazing resistance related genes of Medicago falcata,the Mfβ-Hex1 gene was cloned by PCR,and the bioinformatics of the genes was analyzed by software.The results show that the β-aminohexanidase （Mfβ-Hex1） gene obtained from Medicago falcata was 786 bp,contains an open reading frame of 675 bp,and encodes 224 amino acids,mainly composed of glutamate,valine,leucine,etc.It has high homology with the Mfβ-Hex1 gene in Medicago truncatula and chickpea.Evolutionary information show that Medicago falcata is clustered with Medicago truncatula in the same group.The Mfβ-Hex1 gene belongs to the GH20 family.It doesn′t have a transmembrane region and its secondary structure is mainly α -helix.Its subcellular localization is in the cytoplasm.This study lays a foundation for further research on the function of the Mfβ-Hex1 gene.

Key words: Medicago falcata, Grazing tolerance, Gene clone, Mfβ-Hex1

CLC Number:

S541

XU Wenyi, ZHAO Yan, BAO Jian, WANG Junjie. Cloning and bioinformatics analysis of Mfβ-Hex1 gene in Medicago falcata[J].Journal of Northern Agriculture, 2019, 47(3): 53-57.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.3969/j.issn.2096-1197.2019.03.10

https://journal30.magtechjournal.com/bfnyxb/EN/Y2019/V47/I3/53

References

[1] 韩瑞宏,卢欣石,余建斌,等.苜蓿抗寒性研究进展[J].中国草地学报,2005,27(2):60-65.
[2] 戎郁萍,韩建国,胡跃高,等.放牧苜蓿品种选育研究进展[J].中国草地学报,2004,26(1):50.
[3] 赵爱奇,刘嘉荣,郝金凤,等.甜瓜β-氨基己糖苷酶基因家族的全基因组分析[J].广东农业科学,2014,41(8):185-188.
[4] GAO Y,WELLS L,COMER F I,et al.Dynamic O-glycosylation of nuclear and cytosolic proteins[J].Biol Chem,2001,276 :9838-9845.
[5] LOVE D C,HANOVER J A.The hexosamine signaling pathway:deciphering the“O-GlcNAc code”[J].Sci Signaling,2005,312:13.
[6] SILVERSTONE A L,TSENG T,SWAIN SM,et al.Functional analysis of SPINDLY in gibberellin signaling in Arabidopsis[J].Plant Physiol,2007,143:987-1000.
[7] OLSZEWSKI N,SUN T P,GUBLER F.Gibberellin signaling biosynthesis,catabolism,& response pathways[J]. The Plant Cell Online,2002,14(S1):61-80.
[8] 温都苏,阿拉塔,于斌.内蒙古野生黄花苜蓿种质资源及其开发利用前景[J].畜牧与饲料科学,2004,25(6):72-74.
[9] 曹丽军. β-hex酶在沙红桃果实软化中作用的研究[D].杨凌:西北农林科技大学,2015.
[10] EVA L,CHRISTIANE V,MARTIN P,et al.β-N-Acetylhexosaminidases HEXO1 and HEXO3 Are responsible for the formation of paucimannosidic N-glycans in Arabidopsis thaliana[J].The Journal of Biological Chemistryvol,2011,286:10793-10802.
[11] PRIEM B,GITTI R,BUSH C A,et al.Structure of ten free N-glycans in ripening tomato fruit (arabinose is a constituent of a plant N-glycan)[J].Plant Physiol,1993,102:445-458.
[12] JAGADEESH B H,PRABHA T N,SRINIVASAN K.Activities of β-hexosaminidase and α-mannosidase during development and ripening of bell capsicum (Capsicum annuum var. variata)[J].Plant Sci,2004,167:1263-1271.
[13] WANG J,ZHAO Y,RAY I,et al.Transcriptome responses in alfalfa associated with tolerance to intensive animal grazing[J]. Scientific Reports,2016,6(1):19438.

[1]	LIU Wanpeng, ZHU Lin, LI Yuexuan, LI Zhiyong, GUO Maowei, SUN Juanjuan, LI Hongyan. Effects of planting density on production performance and photosynthetic characteristics of Medicago ruthenica L. [J]. Journal of Northern Agriculture, 2023, 51(2): 65-73.
[2]	ZENG Jijuan, ZHU Qiang, CEN Xiaofei, LI Shuangxi. The physiological response of Lespedeza bicolor seedlings under drought stress [J]. Journal of Northern Agriculture, 2022, 50(2): 53-60.
[3]	LI Hongyan, LI Yuexuan, HU Shun, DUN Huixia. Analysis of stem anatomical structure and evaluation of drought resistance of different strains of Lotus corniculatus [J]. Journal of Northern Agriculture, 2021, 49(4): 70-75.
[4]	GAO Lingzhi;LI Bin;SHI Haibin;QI Yinglong;ZHAO Ju;YIN Chunyan;DI Caixia;LI Yanfang(College of Water Conservancy and Civil Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China;Institute of Resources and Environment and Testing Technology,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;College of Land and Environment,Shenyang Agricultural University,Shenyang 110866,China). Study on the change law of soil temperature of alfalfa (Medicago sativa) in the Ordos Plateau --Taking the Etuoke Banner as an example [J]. Journal of Northern Agriculture, 2019, 47(2): 20-25.
[5]	WU Lan;WU En;QIAN Jiexin;WU Yinga;CHUN Hua(College of Grassland and Resources and Environment,Inner Mongolia Agricultural University,Hohhot 010010,China;Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resoures,Hohhot 010010,China;Zhalantun Meteorologic Bureau,Zhalantun 162650,China). Effects of different amendments on alfalfa growth and arbuscular mycorrhizal symbiosis in saline-alkali soil [J]. Journal of Northern Agriculture, 2019, 47(2): 26-35.
[6]	. Effects of trace elements on the growth and quality of alfalfa in saline soil in Hetao area [J]. Journal of Northern Agriculture, 2017, 45(5): 43-43.
[7]	. Effects of different nitrogen and potassium treatments on the root system of hybrids of caucasian clover×white clover [J]. Journal of Northern Agriculture, 2017, 45(4): 35-35.
[8]	. Principal component and cluster analysis of phenotypic diversity of wild medicago ruthenica germplasm resources in three ecological regions of Inner Mongolia [J]. Journal of Northern Agriculture, 2017, 45(3): 6-6.
[9]	. [J]. Journal of Northern Agriculture, 2017, (1): 42-42.
[10]	. [J]. Journal of Northern Agriculture, 2017, (1): 6-6.
[11]	. Optimization of the fermentation conditions of Alfalfa Rhizobia [J]. Journal of Northern Agriculture, 2016, 44(4): 53-53.
[12]	. Preliminary exploration of fertilization experiment of alfalfa on salt-affected land in Hetao area [J]. Journal of Northern Agriculture, 2016, 44(3): 18-18.
[13]	. [J]. Journal of Northern Agriculture, 2015, 43(2): 36-36.
[14]	. Study on Seed Germination Characteristics of Six Flowers [J]. Journal of Northern Agriculture, 2014, (3): 28-28.
[15]	. Different Application Rate of Rare Earth Influence on Flue-cured Tobacco Production [J]. Journal of Northern Agriculture, 2013, (6): 72-72.

Cloning and bioinformatics analysis of Mfβ-Hex1 gene in Medicago falcata

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0