晋东南旱作区玉米秸秆不同覆盖方式下土壤微生物多样性变化

doi:10.3969/j.issn.2096-1197.2019.04.07

Abstract

Abstract: To investigate the relationship between soil microbes and straw mulching,this study used Illumina Hiseq^TM 2500 high-throughput sequencing technology to determine the sequences of bacterial 16S rRNA V3+V4 and fungal ITS1 region and analyzed the soil microbial bacteria of five different coverage methods in four key growth stages.The results showed that the different mulching of corn stalks had no significant effect on the structure and diversity of bacteria.The treatments showed a trend of an initial decrease,followed by an increase and a decrease in the end;the effect on fungi was significant.Among them,the straw cover of unordered coverage （WX）,whole stalk coverage （ZG） and mulch film coverage （DM） showed a trend of an initial increase first and a following decrease with the growth period.Grinding rotary tillage （FS） showed a trend of initial decreasing,then rising and decreasing at last.The performance of burning stalk （SG） declined slowly.Compared with non-mulching tillage methods,corn straw mulching changes the type and structure of microorganisms in the soil,improves the soil quality,and continues to provide nutrients during the plant growth.

Key words: Straw mulching, Microbial diversity, High-throughput sequencing

CLC Number:

S154.3

LI Wanxing, LI Dan, JIN Kunpeng, LI Xiaoxia, CAO Jinjun, LIU Yongzhong. Changes of soil microbial diversity based on different mulching methods of corn stalk in the dry farming area in Southeast Shanxi Province[J].Journal of Northern Agriculture, 2019, 47(4): 40-46.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.3969/j.issn.2096-1197.2019.04.07

https://journal30.magtechjournal.com/bfnyxb/EN/Y2019/V47/I4/40

References

[1] 郑洪兵,齐华,刘武仁,等.玉米农田耕层现状、存在问题及合理耕层构建探讨[J].耕作与栽培,2014(5):39-42.
[2] 梁卫,袁静超,张洪喜,等.东北地区玉米秸秆还田培肥机理及相关技术研究进展[J].东北农业科学,2016,41(2):44-49.
[3] BAKR N,ELBANA T A,ARCENEAUX A E,et al.Runoff and water quality from highway hillsides:Influence compost/mulch[J].Soil & Tillage Research,2015(150):158-170.
[4] RAM H,DADHWAL V,VASHIST K K,et al.Grain yield and water use efficiency of wheat(Triticum aestivum L.)in relation to irrigation levels and rice straw mulching in North West India[J].Agricultural Water Management,2013,128:92-101.
[5] 王小果. 浅述秸秆直接还田技术[J].农业技术与装备,2017(10):53-55.
[6] 王文明. 秸秆还田的利弊浅析[J].南方农业,2017,11(34):103-105.
[7] 张星杰,刘景辉,李立军,等.保护性耕作对旱作玉米土壤微生物和酶活性的影响[J].玉米科学,2008,16(1):91-95.
[8] FREY S D,SIX J,ELLIOTT E T.Reciprocal transfer of carbon and nitrogen by decomposer fungi at the soil-litter interface[J].Soil Biology and Biochemistry,2003,35(7):1001-1004.
[9] HOLLAND E A,COLEMAN D C.Litter placement effects on microbial and organic matter dynamics in an agroecosystem[J].Ecology,1987,68(2):425-433.
[10] MARY B,RECOUS S,DARWIS D,et al.Interactions between decomposition of plant residues and nitrogen cycling in soil[J]. Plant and Soil,1996,181(1):71-82.
[11] 徐广,刁治民,曾智科,等.微生物降解秸秆的研究进展与前景展望[J].科技资讯,2007(22):137.
[12] MARSCHNER P,UMAR S,BAUMANN K.The microbial community composition changes rapidly in the early stages of decomposition of wheat residue[J]. Soil Biology and Biochemistry,2011,43(2):445-451.
[13] BARDGETT R D,FRANKLAND C J,WHITTAKER J B.The effects of agricultural management on the soil biota of some upland grasslands[J]. Agric Ecosyst Environ,1993,45(1/2):25-45.
[14] CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community seq-uencing data[J].Nature Methods,2010,7(5):335-336.
[15] EDGAR R C.Search and clustering orders of magnitude faster than BLAST[J]. Bioinformatics,2010,26(19):2460-2461.
[16] WANG Y,SHENG H F,HE Y,et al.Comparison of the levels of bacterial diversity in freshwater,intertidal wetland,and marine sediments by using millions of illumina tags[J].Appl Environ Microbiol,2012,78(23):8264-8271.
[17] 袁红朝,吴昊,葛体达,等.长期施肥对稻田土壤细菌、真菌多样性和群落结构的影响[J].应用生态学报,2015,26(6):1807-1813.
[18] JANGID K,WILLIAMS M A,FRANZLUEBBERS A J,et al.Relative impacts of land-use,management intensity and fertilization upon soil microbial community structure in agricultural systems[J].Soil Biology and Biochemistry,2008,40(11):2843-2853.
[19] 胡宜刚,叶震,陈秀蓉.高寒草地土壤优势细菌在草地物质循环中的作用初探[J].草原与草坪,2008(2):27-30.
[20] BARNS S M,TAKALA S L,KUSKE C R.Wide distribution and diversity of members of the bacterial kingdom acidobacterium in the environment[J].Applied and Environmental Microbiology,1999,65(4):1731-1737.
[21] WISE M G,MCARTHUR J V,SHIMKETS L J.Bacterial diversity of a carolina bay as determined by 16S rRNA gene analysis:confirmation of novel taxa[J].Appl Environ Microbiol,1997,63(4):1505-1514.
[22] 朱兆香. 土壤放线菌的筛选、鉴定及其活性代谢产物的研究[D].哈尔滨:东北农业大学,2011.
[23] DEBRUYN J M,NIXON L F,FAWAZ M N,et al.Global biogeography and quantitative seasonal dynamics of gemmatimonadetes in soil[J]. Appl Environ Microbiol,2011,77(17):6295-6300.

Changes of soil microbial diversity based on different mulching methods of corn stalk in the dry farming area in Southeast Shanxi Province

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Metrics

Comments

Recommended 0

[1]	CHENG Zhipeng, ZHANG Chengze, WANG Fugui, WANG Zhen, ZHANG Yuezhong, YAN Liwei, LIANG Hongwei, YANG Zhihong, GAO Julin, WANG Zhigang. Effects of tillage methods on Inner Mongolia dry farming regions soil moisture,temperature and maize yield [J]. Journal of Northern Agriculture, 2023, 51(2): 22-32.
[2]	ZHU Jinfeng;FAN Zuqing;CHEN Qilong;WANG Jieqiong;CHEN Chong;LU Aqian;LI Hongli;WANG Yan(Luohe Tobacco Company of Henan Province,Luohe 462000,China;College of Chemical Engineering and Energy,Zhengzhou University,Zhengzhou 450001,China). Effects of continuous cropping years on soil microbial flora in the tobacco rhizosphere [J]. Journal of Northern Agriculture, 2019, 47(1): 42-48.
[3]	. Effect of Different Coverage Cultivation Mode on Growth, Quality and Yield of the Flue-cured Tobacco [J]. Journal of Northern Agriculture, 2014, (2): 28-28.