Abstract: Ninety-six strains of Trichoderma were isolated from one hundred seventy samples of soil and other materials collected from fourteen regions in Ningxia. The morphological identification work indicated that they belonged to nine species of Trichoderma and two unidentified Trichoderma species,namely Trichoderma harzianum, T.viride, T.longibrachiatum, T.koningii, T.pseudokoningii ,T.fertile ,T.aureoviride ,T.atroviride,T.hamatum,T.spl and T.sp2.The antagonism and antibacterial activity test result showed that there were different antagonisms between eleven Trichoderma species and Botrytis cinerea in grap, tomato and cucumber,especially,inhibition of Trichoderma harzianum,T.viride,T.aureoviride,T.hamatum and T.spl.were more obvious. Their growth rates are 1.1-3.0 times faster than Botrytis Cinerea Pers sp.Their inhibition index and inhibition rates repectively reached Ⅰ or Ⅱ and 98.78%.