Abstract: We studied the genetic diversity of 35 hulled oat accessions using SSR and SRAP markers in this paper. Nineteen SSR primer pairs and 26 SRAP primer combinations were used, and 32 and 259 loci were amplified, of which 30 （93.8%） and 199 （76.8%） were polymorphic, respectively. The SSR makers alleles polymorphism information content（PIC） ranged from 0.665 4 to 0.798 5 ,with an average of 0.755 6. The effective number of alleles for each locus （Ne） varied from 1.159 2 to 1.971 1,with an average of 1.751 4.The Shannon-Weaver diversity index （H） ranged from 0.2643 to 0.8362 and averaged 0.596 9.The SRAP makers alleles polymorphism information content（PIC） ranged from 0.354 6 to 0.932 1, with an average of 0.743. The effective number of alleles for each locus （Ne） varied from 1.102 4 to 1.992 8, with an average of 1.672 4. The Shannon-Weaver diversity index （H） ranged from 0.168 2 to 0.691 4 and averaged 0.555 6. The genetic similarity coefficient ranged from 0.46 to 0.95. The 35 materials could be clustered into 4 groups at the genetic similarity coefficient 0.70. Accessions of the same origin were in more than one group, it indicating that abundant genetic diversity existed in the hulled oat materials from Chifeng. The results indicated that the work of hulled oat resources collection, reservation, identification and utilization in Chifeng should be strengthened.