Abstract: The aim was to research the rapid propagation of phalaenopsis amabilis,especially the optimum formula of culture medium and culture procedure at each phase were researched by taking peduncles after flowering period as explants.The results showed that:the optimum medium for inducing the peduncles of phalaenopsis amabilis was 1/2MS+6-BA2.5 mL+NAA0.5 mL;the optimum medium for proliferation coefficient of phalaenopsis amabilis was MS+6-BA5.0 mL+NAA0.5 mL;the optimum medium for rooting of phalaenopsis amabilis was 1/2MS contained 10%banana puree.After training,the cultured plantlets was transplanted in wet algae and keep the humidity above 85%.