Abstract: Lilium longiflorum as the research object, with MS as the basic culture medium,optimize the lilies explant best way to sterilization, eventually choose 5% concentration of sodium hypochlorite, sterilization time for 10 rain. Based on 8 additional different concentrations of 6 - BA, NAA of MS culture medium, according to a study in MS＋I mg/mL 6-BA＋0.1 mg/mL NAA medium lilies proliferation effect was good, proliferation coefficient of 6.88. Sucrose concentration was 60%, the best bulb effect in vitro, the produce rate of bulbs produce bulbs rate of 100%, bulb was 1.12 cm in diameter, mass was 2.26 g. In 1/2 MS ＋ 0.5 mg/mLNAA medium to take root, rooting rate of 100%, average root length was 2.83 cm. In the end, getting the tube seedling transplanting, the survival rate was 83%.We established Lilium longiflorum system of tissue culture technology and provided certain technical guidance for chifeng area.