Abstract:

【Objective】To establish in vitro rapid propagation system of edible lily.【Methods】Using tissue culture method with edible lily N82 and N136 bulbs as explants，the optimal disinfection and sterilization methods，the optimal adventitious bud induction，proliferation and rooting medium, and the best transplantation substrate for tissue culture seedlings were chosen.【Results】The disinfection and sterilization methods of the two edible lily cultivars were both immerse the explants in 75% ethanol for 30 s then treated with 3% NaClO solution for 10 min. The optimal adventitious bud induction medium for N82 was MS plus 0.5 mg/L 2，4-D and 1.0 mg/L 6-BA，with induction rate at 90.00%. The optimal proliferation medium was MS plus 0.3 mg/L NAA and 0.3 mg/L TDZ，with proliferation coefficient at 5.66. The optimal rooting medium was MS plus 2.0 mg/L NAA，MS plus 2.0 mg/L IBA and MS plus 0.3 g/L AC，all with rooting rate at 100%. The optimal transplantation substrate for tissue culture seedlings was 1:1 mixture of turf soil : river sand，with 100% survival rate and good seedlings growth. The optimal adventitious bud induction medium for N136 was MS plus 1.0 mg/L NAA and 1.0 mg/L 6-BA，with induction rate at 93.30%. The optimal proliferation medium was MS plus 0.5 mg/L NAA and 0.2 mg/L TDZ，with proliferation coefficient at 5.86. The optimal rooting medium was MS plus 0.5 g/L AC，with rooting rate at 100%. The optimal transplantation substrate for tissue culture seedlings was 1:1:1 mixture of turf soil:perlite:river sand，with 100% survival rate.【Conclusion】 For the cultivar N82，the optimal adventitious bud induction medium was MS plus 0.5 mg/L 2，4-D and 1.0 mg/L 6-BA，the optimal proliferation medium was MS plus 0.3 mg/L NAA and 0.3 mg/L TDZ，the optimal rooting medium was MS plus 2.0 mg/L NAA，MS plus 2.0 mg/L IBA and MS plus 0.3 g/L AC，and the optimal transplantation substrate for tissue culture seedlings was 1:1 mixture of turf soil : river sand. For the cultivar N136，the optimal adventitious bud induction medium was MS plus 1.0 mg/L NAA and 1.0 mg/L 6-BA，the optimal proliferation medium was MS plus 0.5 mg/L NAA and 0.2 mg/L TDZ，the optimal rooting medium was MS plus 0.5 g/L AC，and the optimal transplantation substrate for tissue culture seedlings was 1:1:1 mixture of turf soil:perlite:river sand.

Key words: Edible lily, Tissue culture, Plant growth regulator, Rapid propagation system