水稻藤仓镰孢菌对氰烯菌酯的抗药性分析

doi:10.12190/j.issn.2096-1197.2020.06.13

Abstract

Abstract: 【Objective】To determine the phenamicril resistance of Fusarium fujikuroi in rice and its resistance mechanisms.【Methods】The minimum inhibitory concentration(MIC)of phenamacril was tested from 86 Fusarium fujikuroi strains obtained from Suqian area of Jiangsu Province,two resistant strains and two sensitive strains were selected to test their virulence,growth rate and spore output in laboratory. The sequence of myosin5 gene was also analyzed.【Results】MIC of 68% Fusarium fujikuroi strains was >5 μg/mL to phenamicril,and strains Sq6 and Sq12 were resistant and Sq15 and Sq20 were sensitive,there was no significant difference in mycelial growth rate(P>0.05),and the spore output of Sq12 and Sq20 was significantly higher than that of Sq6 and Sq15(P<0.05). According to the sequence alignment of myosin5 gene,the resistant strains(Sq6,Sq12)mutated from arginine to serine at codon 326.【Conclusion】The resistance frequency of Fusarium fujikuroi in rice to phenamicril was 68% in Suqian. When phenamicril was used for seed dressing,the frequency and dosage should be considered in order to reduce or delay the development of resistance.

Key words: Rice, Fusarium fujikuroi, Phenamacril, Resistance, myosin5

CLC Number:

S511

ZHANG Zhihui, JIN Qian, WANG Xiawen, WANG Feifei, WANG Xinhai. Research on the resistance of Fusarium fujikuroi in rice to phenamacril[J].Journal of Northern Agriculture, 2020, 48(6): 81-86.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2020.06.13

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Research on the resistance of Fusarium fujikuroi in rice to phenamacril

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