基于农杆菌介导法将CP4-EPSPS基因转入玉米自交系B73的研究

doi:10.12190/j.issn.2096-1197.2023.01.04

Abstract

Abstract: 【Objective】Establish a maize genetic transformation system to cultivate maize lines with herbicide tolerance and address the issue of weed damage in maize.【Methods】Herbicide tolerance gene（CP4-EPSPS）was transformed into immature embryos of maize inbred line B73 by agrobacterium mediated transformation. The transgenic plants were identified by PCR and qRT-PCR. Low copy transgenic events were screened by ddPCR,followed by phosphinothricin tolerance test.【Results】12 of the 181 acquired tolerance lines tested positive. The exogenous herbicide tolerance gene（CP4-EPSPS）of the transgenic plants expressed normally at the transcriptional level. Five low copy lines were selected from the transgenic plants. All the transgenic plants were phosphinothricin tolerant in the tolerance test. T₁ seeds from the 5 transgenic low copy lines were harvested.【Conclusion】Established a maize genetic transformation system mediated by Agrobacterium transformation using B73.

Key words: Maize, Agrobacterium mediate transformation, Glyphosate resistance gene（CP4-EPSPS）, Genetic transformation

CLC Number:

S513.032

HAN Ping′an, CHANG Yue, TANG Kuan′gang, LI Xiaodong, WANG Liwei, LIANG Yahui, YANG Jing, SHI Haibo, WU Xinrong. Study on agrobacterium mediated transfer of CP4-EPSPS gene into maize inbred line B73[J].Journal of Northern Agriculture, 2023, 51(1): 31-37.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2023.01.04

https://journal30.magtechjournal.com/bfnyxb/EN/Y2023/V51/I1/31

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Study on agrobacterium mediated transfer of CP4-EPSPS gene into maize inbred line B73

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