PCR快速检测种子携带绿豆晕疫病菌方法的应用

doi:10.12190/j.issn.2096-1197.2021.05.15

Abstract

Abstract: 【Objective】 Using PCR to rapidly screen primers with high sensitivity to the Pseudomonas savastanoi pv. phaseolicola on mung bean seed surface. Provide technical support for the detection of seed-carrying Pseudomonas savastanoi pv. phaseolicola in mung bean. 【Methods】 Seven pairs of specific primers were selected to perform a series of PCR reactions on Pseudomonas savastanoi pv. phaseolicola suspensions at different concentrations,and their sensitivity was verified. 【Results】 Three pairs of primers with high sensitivity were selected. The simulated seed surface bacteria detection results showed that the minimum concentration of bacterial suspension detected by primer B4-1-F/B4-1-R and B4-2-F/B4-2-R was 1×10⁴ CFU/mL,and the minimum concentration of bacterial suspension detected by primer HB14F/HB14R was 1×10⁶ CFU/mL. The results showed that primer B4-1-F/B4-1-R and B4-2-F/B4-2-R could detect the seed sample with seed-carrying rate of 0.062 5% after shaking and washing for 1 h,and primer HB14F/HB14R could detect the seed sample with seed-carrying rate of 0.125% after shaking and washing for 4 h. 【Conclusion】 PCR could be used to rapidly detect seed carrying the Pseudomonas savastanoi pv. phaseolicola on mung bean seed surface,which was convenient and quick.

Key words: Mung bean, Halo blight, PCR, Seed-carrying, Primer

CLC Number:

S432.4

TIAN Xiaoyan, HE Xiaoyong, KONG Qingquan, ZHAO Cunhu, CHEN Wenjin, LI Zhidong, JIA Zhuanqing. Rapid detection of seed carrying Pseudomonas savastanoi pv. phaseolicola in mung beans using PCR[J].Journal of Northern Agriculture, 2021, 49(5): 106-111.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2021.05.15

https://journal30.magtechjournal.com/bfnyxb/EN/Y2021/V49/I5/106

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Rapid detection of seed carrying Pseudomonas savastanoi pv. phaseolicola in mung beans using PCR

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