【Objective】 The high yield and adaptability of varieties（lines）participating in the naked oats regional test in China from 2003 to 2014 were determined,and suitable planting sites were devided to provide a basis for the promotion of the varieties. 【Methods】 The standard deviation of means（SD）corrected GGE-Biplot（SD-GGE）method was used to evaluate the high yield and adaptability of 4 rounds of trials and 30 naked oat varieties in 21 pilots. 【Results】 The analysis of variance showed that the environment was the main factor affecting yield changes,accounted for 47.53%-83.95% of the total variation. On the GGE-Biplot,9418,Bayou No.10,Mengyan No.2 and Zhangyou No.7 were higher yields in most of the pilots tested. In all 4 rounds of trials,the high yield of all varieties in the Wuzhai County in Shanxi Province was basically the same as the order of the average yield of all the experimental sites. 【Conclusion】 9418,Bayou No.10,Mengyan No.2 and Zhangyou No.7 were high yield and strong adaptability varieties. Wuzhai County in Shanxi Province was a good respresentative of all the sites.

【Objective】 To make full use of potato germplasm resources and provide theoretical basis for potato variety creation,the genetic diversity of 30 collections was evaluated. 【Methods】 The genetic diversity evaluation of 30 potato germplasm resources were analyzed and evaluated by the difference,genetic diversity index,principal component analysis,correlation analysis and cluster analysis. 【Results】 The diversity index of quality traits of potato varieties（lines）ranged from 0.673 to 1.424. The diversity index of leaf color,flesh color and tuber shape was higher. The diversity index of quantitative traits ranged from 1.705 to 1.997,with the commercial potato rate being the highest and the number of main stems being the lowest. Principal component analysis showed that the cumulative contribution rate of the first four principal components was 75.989%,which could cover information for most characters. Correlation analysis showed that plant height was positive（P<0.05）correlated with the rate of commercial potato and yield. 30 potato germplasm resources were divided into 3 groups using cluster analysis. Group Ⅰ contained 8 materials,group Ⅱ contained 9 materials,and group Ⅲ contained 13 materials. 【Conclusion】 The 30 potato germplasm resources were classified into 3 major groups,with rich variation and wide genetic diversity.

【Objective】 To screen buckwheat varieties（lines）suitable for different growing areas in Inner Mongolia and to meet the needs of buckwheat processing companies,as well as,to provide reference for buckwheat variety evaluation and application. 【Methods】 The AMMI model was used to analyze the stable yield and high yield of 15 buckwheat varieties（lines）in 4 pilots（the experimental site in Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Wuchuan County of Hohhot,Guyang County of Baotou,Naiman Banner of Tongliao）. 【Results】 In varince analysis,variety,experimental location and the interaction between the variety and experimental location were all extremely significant（P<0.01）. According to AMMI model analysis,the interaction effect of IPCA1 and IPCA2 explained 92.28% of the variety and location interaction information which indicated the varieties and locations chosen were representative. Mengku 11,Mengku 09,Mengku 93,Mengku 13,Mengku 06 were high and stable yield varieties（lines）,and Mengku 45,Mengku 66 were stable varieties with poor yield,while Mengku 87 and Mengku 53 were high yield but poor stability. Guyang County of Baotou had the highest resolution for the variety identification abilities,while Wuchuan County of Hohhot had the weakest in the 4 pilots. 【Conclusion】 Mengku 11,Mengku 09,Mengku 93,Mengku 13,Mengku 06 were suitable for large-scale cultivation in Inner Mongolia. The AMMI model could be used to accurately analyse variety stability and high yield.

【Objective】 To study the color wheat tissue culture regeneration system and provide a theoretical basis for establishing a high-frequency color wheat genetic transformation system. 【Methods】 The mature embryos of three color wheat varieties（lines）of Zhongheimai No.6,CM-Z2 and Zhonglanmai No.1 were used. The induction status was recorded at 8,14,and 20 days after callus induction. Growth regulator IAA+KT and IAA+6-BA combination were used to redifferentiate color wheat callus. The callus rate,differentiation rate and seedling rate of different treatments were evaluated. 【Results】 The explants of Zhongheimai No.6,CM-Z2 and Zhonglanmai No.1 had the best callus induction effect after cultured for 14 days,with callus rates ranging from 80.54% to 95.39%. The regeneration ability of color wheat mature embryo callus was related to genotype and culture medium. Zhongheimai No.6 had the best regeneration ability,with a differentiation rate of 87.68%-97.50%,and a seedling rate of 17.26%-61.73%. The seedling rate was higher in the differentiation medium containing 0.1 mg/L IAA and 0.5 mg/L 6-BA,0.5 mg/L IAA and 4 mg/L KT,with a rate of 61.73% and 56.25% respectively. CM-Z2 followed Zhongheimai No.6,had a differentiation rate of 60.23%-95.45% and seedling rate of 2.27%-34.09%. CM-Z2 had the highest seedling rate in the differentiation medium containing 0.5 mg/L IAA and 4 mg/L KT,reaching 34.09%. 【Conclusion】 Zhongheimai No.6 was an ideal material for color wheat genetic transformation. Regeneration medium containing 0.1 mg/L IAA and 0.5 mg/L 6-BA,0.5 mg/L IAA and 4 mg/L KT were suitable for Zhongheimai No.6 and CM-Z2.

【Objective】 To explore the photosynthetic characteristics of Cyperus esculeutus under density-sowing date coupling in the central and western regions of Inner Mongolia,and to determine the appropriate sowing time and density for the cultivation of Cyperus esculeutus. 【Methods】 The experiment was carried out in the experimental base of Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences. Fengchan No.2 was used as the test material with the split zone design,two sowing dates（S1 on April 30;S2 on May 10）and 3 densities（60 000 plants/hm² named D1;120 000 plants/hm² named D2;180 000 plants/hm² named D3）.The daily changes of net photosynthetic rate（Pn）,transpiration rate（Tr）,stomatal conductance（Gs）and intercellular CO₂ concentration（Ci）of the main stem leaves of Cyperus esculeutus at different growth periods were analysed. 【Results】 The diurnal variation trend of Pn,Tr and Gs of Cyperus esculeutus under the density-different sowing dates coupling was “M” shape,and the double peak values of Pn,Tr and Gs were at 10：00 and 13：00,10：00 and 14：00,12：00 and 14：00 at tuber primary stage,tuber swelling stage and tuber maturity stage,respectively. The diurnal variation of Ci presented a“U” shape distribution with the peak values at 8：00 and 18：00. The diurnal variation of Pn,Tr and Gs were all expressed as the tuber primary stage>tuber swelling stage>tuber maturity stage at the beginning of tuber. The daily average Pn and Gs were the highest in S2D3 treatment and significantly different from other treatments（P<0.05）,they were 16.086 7 μmol/（m²·s）,0.148 0 mol/（m²·s）respectively;the daily average Tr of S2D3 at tuber primary stage was the largest and significantly different from that of S1D3,S1D2 and S2D2（P<0.05）,at 0.003 7 mol/（m²·s）;Ci trough value appeared at 12：00 in tuber primary stage,13：00 at the swelling stage and maturity stage of tuber. The daily average Ci was the highest in S2D3 treatment at tuber primary stage and was significantly different from S1D1,S1D2 and S2D2 treatments（P<0.05）,at 142.959 6 μmol/mol. 【Conclusion】 The most suitable sowing date of Cyperus esculentus in central and western Inner Mongolia was mid-May with the density 180 000 plants/hm².

【Objective】 To explore the optimum cultivation techniques for the production of edible sunflower seed under natural pollination conditions in Gansu. 【Methods】 Sterile line 9063A and restorer line A1361R of hybrid ZH9018 were used as test materials. With a split zone design,the main zone was designed for testing the planting density with four levels：34 500,40 500,46 500 and 52 500 plants/hm². The auxiliary zone was for fertilizer with three levels set on top of base compound fertilizer of 450 kg/hm². The three levels were,150 kg/hm² of urea topdressing at both squaring stage and filling stage;150 kg/hm² urea topdressing plus 45 kg/hm² potassium sulfate at squaring stage and 150 kg/hm² urea topdressing at filling stage;150 kg/hm² urea topdressing plus 45 kg/hm² potassium sulfate at both squaring stage and filling stage. Relevant traits were then investgated. Yield was calcuated during the harvest period. Seeds graded after the test. 【Results】 Different planting density had a significant effect on edible sunflower stem thickness,disk diameter,single disk seed weight,100-grain weight,and seed size（P<0.05）. The effect of different fertilization treatments on plant height,stem thickness,disk diameter and single disk seed weight and 100-grain weight was not significant（P>0.05）. The effect of planting density on the seeds yield was extremely significant（P<0.01）;the effect of different fertilization treatments on seeds yield was not significant（P>0.05）. The optimal condition was 150 kg/hm² urea topdressing plus 45 kg/hm² potassium sulfate at both squaring stage and filling stage,and planting density at 46 500 plants/hm² was the best seed production,with seed yield reached 2 333.1 kg/hm²;the highest seed yield of M-level seeds at 82.9%. 【Conclusion】 Planting density of 46 500 plants/hm²,base compound fertilizer 450 kg/hm²,squaring stage and filling stage 150 kg/hm² urea topdressing plus 45 kg/hm² potassium sulfate was recommended for edible sunflower seed production in Gansu.

【Objective】 To provide references for the cultivation and demonstration of ornamental sunflowers in coastal areas of Jiangsu. 【Mothods】 Self-selected ornamental sunflower Yankui No.3,Yankui No.4 and Yankui No.5 were tested using a random block design. The flowering characteristics of the ornamental sunflowers and the relationship with sowing date were investigated by setting two sowing dates in summer and autumn. 【Results】 Sowing dates and varieties had significant effects on the initial flowering stage,flowering time and flowering ratio of discs on secondary branches of ornamental sunflower（P<0.05）. Compared with summer sowing,autumn sowing significantly prolonged individual flower trays,individual plants and the flowering period of population（P<0.05）.Yankui No.3 had the longest flowering time in autumn,with 29.9 and 38.6 flowering days per plant and population,respectively. The flowering order of ornamental sunflowers was generally shown as the main disc first,followed by the main discs of side branches,which bloomed from top to bottom,then starting with inverted 1 inverted 2.The ratio of discs on the secondary branches varied according to the sowing date,variety and location of the lateral branches. Overall the ratio on summer sowing was higher than autumn sowing,and the ratio on inverted 1 branches was higher than the inverted 2 branches. 【Conclusion】 Sowing Yankui No.3 in autumn in the coastal areas of Jiangsu could fully realize its ornamental potential.

【Objective】 To determine the effects of different application methods of uniconazole on the yield and quality of edible sunflowers,in order to solve the problems of the declining in the commodity and quality of edible sunflower seeds in Hetao Irrigation Area. 【Methods】 A random block design was used to examine the effects of different concentrations and application methods of uniconazole on edible sunflower yield,grain apparent traits,and kernel nutritional quality using two ways,seed soaking and foliar spraying with uniconazole. 【Results】 Both seed soaking and foliar spraying with uniconazole increased the 100-grain weight,kernel rate and yield of edible sunflower. The best yield performance was when the concentration of seed soaking treatment was A4（330 mg/L）and the concentration of foliar spraying was B4（400 mg/L）. The yield increased by 15.62% and 16.20% respectively compared with the control. When seed soaking treatment concentration was A2（230 mg/L）and the foliar spraying treatment concentration was B4（400 mg/L）,the grain width increased by 10.42% and 12.50%,respectively,compared with the control. The difference was extremely significant（P<0.01）.When the concentration of seed soaking treatment was A4（330 mg/L）,the crude protein content of seed kernel had the highest increase,which was 9.49% higher than the control. The difference was extremely significant（P<0.01）. When the concentration of foliar spraying treatment was B4（400 mg/L）,the crude fat content of seed kernels had the highest increase,which was 16.18% higher than the control. The difference was extremely significant（P<0.01）. The content of essential amino acids increased when the concentration of seed soaking treatment was A2（230 mg/L）,A3（280 mg/L）,and A4（330 mg/L）.When the foliar spraying treatment was B3（350 mg/L）and B4（400 mg/L）the valine content increased. The methionine content increased at each concentration of the seed soaking treatment and the foliar spraying treatment. And the lysine content increased at the B2（300 mg/L）,B3（350 mg/L）and B4（400 mg/L）treatment. 【Conclusion】 Both seed soaking and foliar spraying with uniconazole increased the yield of edible sunflower and improved the quality of grains. The results showed that seed soaking treatment A4（330 mg/L）and foliar spraying treatment B4（400 mg/L）were the best concentrations in edible sunflower seed appearance,yield,nutrient quality and essential amino acid content.

【Objective】 To explore the salt-reducing effect of microbial fertilizer to different degrees of saline soil and the growth response of winter wheat. 【Methods】 Coastal saline soil was used to create mildly,moderately,and heavily salined soils.Yi'an improved soil remedial 2 bacteria agent was selected. Fertilization gradient of 750,1500 and 2250 kg/hm² were set to compared and analyze soil salt content,winter wheat plant height,SPAD value,fresh weight,root length. 【Results】 The application of microbial bacterial fertilizers to different degrees of saline soil had some salt-reducing effect,and the soil salt content decreased by 6.02%-36.27% compared with the control. The mildly salinization treatment with a microbial fertilizer application rate of 1500 kg/hm² had the most obvious salt reduction effect. Application of microbial fertilizer treatment compared with the control,the plant height of winter wheat increased by 18.01%-33.52%,SPAD value increased by 9.82%-24.30%,fresh weight increased by 10.53%-38.46%,and root length increased by 14.08%-32.19%. When the amount of microbial fertilizer was less than 1500 kg/hm²,the salt-reducing effect increased with the increase of the application amount,and the growth of winter wheat increased significantly. When the amount was more than 1500 kg/hm²,the salt-reducing effect increased slowly. 【Conclusion】 Application of microbial fertilizer in salinized soil of different degrees had obvious salt-reducing effect and significantly promoted the growth of winter wheat.

【Objective】 To study the effects of foliar spraying phosphorus,potassium and boron fertilizers on broad bean yield,and provide a basis for the application of foliar fertilizers in broad bean production. 【Methods】 LX-17 broad bean was used as test material. 4 treatments,boron（B）,potassium dihydrogen phosphate（PK）,boron and potassium dihydrogen phosphate combined application（PKB）and no fertilizer（CK）were applied to broad bean once at budding stage,initial flowering stage and final flowering stage. The agronomic traits,fresh bean yield,dry bean yield,and seed quality of broad bean under each treatment condition were determined. The correlation analysis between each trait and the yield of fresh and dry beans was carried out. 【Results】 Both PK and PKB treatments increased broad bean stem thickness while lowering plant height and internode length. B treatment decreased the plant height and stem thickness when high concentration boron fertilizer was used,which was significantly different from CK（P<0.05）. All three foliar spraying treatments increased the effective branch number. PKB treatment increased the number of fresh and dry pods per branch of broad bean,decreased the dry weight of 100 seeds,and was significantly different compared with CK（P<0.05）. The number of fresh and dry pods per branch of broad bean decreased as the concentration increased in the B treatment,whereas the number of seeds per pod increased. B_0.10 treatment had the best result. Correlation analysis of the yield and other indicators showed that yield was significantly or extremely significantly positive correlated with stem diameter,number of seeds per pod,number of fresh pods per branch,and fresh weight of 100 seeds. Compared with CK,PK and PKB treatments had no significant effect on the germination rate and germination potential of broad bean seeds（P>0.05）. PK treatment increased the first grade rate of broad bean seeds. High concentration of B treatment decreased the germination rate,potential germination rate and first-class rate. 【Conclusion】 Spraying potassium dihydrogen phosphate,low-concentration boron fertilizer,and foliar fertilizer combined with potassium dihydrogen phosphate and boron fertilizer during the budding stage,initial flowering stage and final flowering stage could promote the broad bean growth and increase the yield. The combined application of 0.3%-0.4% potassium dihydrogen phosphate and 0.1% boron had the best treatment effect.

【Objective】 To explore the effects of sheep dung anaerobic and aerobic composting extracts on tomato yield,quality and soil nutrient content in greenhouse. 【Methods】 To compare and analyze tomato plant traits,fruit traits,yield,quality and soil nutrient content in greenhouse,5 treatments were conducted,no fertilizer（CK）,organic fertilizer（F1）,sheep dung anaerobic composting extract（F2）,sheep dung aerobic composting extract（F3）,and single fertilizer（F4）. 【Results】 Plant height（173.60 cm）,stem diameter（16.48 cm）,fruit transverse diameter（78.02 mm）,fruit longitudinal diameter（57.68 mm）,average single fruit weight（0.20 kg）,yield（138 000 kg/hm²）,fruit soluble total sugar content（6.05%）,center sugar content（5.50%）in F2 treatment were significantly different from other treatments（P<0.05）,and the soil organic matter content（71.00 g/kg）,total nitrogen content（3.55 g/kg）,hydrolyzable nitrogen content（262.00 mg/kg）,available phosphorus content（277.60 mg/kg）,available potassium content（535.00 mg/kg）,the total amount of water-soluble salt（0.70 g/kg）were also significantly different from other treatments（P<0.05）. Leaf width（15.20 cm×8.50 cm）,plant width（50.00 cm×55.00 cm）,relative chlorophyll content（73.45%）and fruit VC content（27.60 mg per 100 g fresh fruit）in F3 treatment were significantly different from other treatments（P<0.05）,and the total potassium content of the soil（26.00 g/kg）and pH value（7.91）were also significantly different from other treatments（P<0.05）. Compared with other treatments,the total acid content（4.80 g/kg）and soluble solid content（7.50%）of tomato in F1 treatment were significantly different（P<0.05）,and the soil total phosphorus content（1.66 g/kg）was also significantly different from other treatments（P<0.05）. 【Conclusion】 After applying sheep dung anaerobic composting extract and aerobic composting extract,greenhouse tomatoes grew better,yield increased,and quality improved. The composts also had a positive effect on the improvement of soil nutrients in the greenhouse.

Saline-alkali land is an important reserve cultivated land resource in China. The development and utilization of saline-alkali cultivated land has important practical significance for meeting population and food needs of as well as agriculture long-term sustainablility. Straw returning can improve soil structure and fertility,improve the quality of cultivated land,and reduce the salt accumulation in topsoil,making it an effective measure for improving saline-alkali soil. This paper summarized the current state of domestic straw returning technology,explained the principle and application of straw returning technology to improve saline-alkali soil,and discussed the effects of improvement on saline-alkali soil from the aspects of soil physical properties,chemical properties,biological characteristics and crop yield. The impact of improved saline-alkali straw returning technology application foreground was prospected.

【Objective】 To clarify the distribution of fusarium head blight resistance genes Fhb1 and Fhb7 in Huaibei wheat,in Jiangsu Province. To provide a basis for breeding resistance lines to fusarium head blight. 【Methods】 In 155 novel wheat lines from Huaibei,the genotypes of diagnostic molecular markers Fhb1 and dominant molecular markers Fhb7 of wheat main fusarium head blight resistance genes were analysed. 【Results】 Resistance genes Fhb1 and Fhb7 were both distributed in the 155 novel wheat lines. The frequency of Fhb1 was higher than that of Fhb7. Among the 4 genotypes,fhb1/Fhb7 gene combination did not appear,151 lines containing fhb1/fhb7 gene combination,accounted for 97.4% of the total,3 lines containing Fhb1/fhb7 gene combination,accounted for 1.9%. There was only one line with Fhb1/Fhb7 genotype containing the two resistance genes,accounted for 0.7%. 【Conclusion】 The Fhb1 and Fhb7 genes were less distributed in the novel wheat lines in Huaibei,Jiangsu Province.

【Objective】 Isolation and identification of endophyte from the roots of Tetraena mongolica,screening for antibacterial activity,and analyzing their growth-promoting effects on seeds and plants of Tetraena mongolica. 【Methods】 The endophyte was isolated and purified from Tetraena mongolica roots using Gao's No.1 medium containing potassium dichromate. The strains were identified by physiological and biochemical characteristics and the construction of a phylogenetic tree of 16S rRNA gene sequence. A comparative test was used to detect the growth-promoting activity of the strain on Tetraena mongolica seeds and plants. Plate confrontation method was used to detect the antagonistic activity of the strain. 【Results】 The endophyte isolated from the root of Tetraena mongolica was an actinomycete strain named RE4. After identification,the strain RE4 was 100% similar to the model strain Streptomyces canus CSSP527^T,and the evolutionary distance was relatively close to the model strain,which was Streptomyces Streptomyces canus. RE4 could effectively promote the germination and seedling growth of Tetraena mongolica,the plant height,root length,rooting rate and germination rate of Tetraena mongolica seedlings treated with RE4 increased by 27.89%,197.09%,26 percentage points and 4 percentage points respectively compared with the control. RE4 could inhibit the growth of Verticillium dahliae,Fusarium oxysporum,Sclerotinia sclerotiorum and Phytophthora infestans,and the inhibition rates were 68.4%,63.8 %,53.8% and 38.6%. The inhibitory diameters of RE4 against Saccharomyces cerevisiae,Bacillus subtilis,Staphylococcus aureus and Escherichia coli were 17.04,12.45,11.39,11.35 mm,respectively. 【Conclusion】 The endophyte isolated from the roots of Tetraena mongolica were identified as Streptomyces Streptomyces canus,which could antagonize some pathogens and promote the germination of seeds and the growth of seedlings of Tetraena mongolica.

【Objective】 Using PCR to rapidly screen primers with high sensitivity to the Pseudomonas savastanoi pv. phaseolicola on mung bean seed surface. Provide technical support for the detection of seed-carrying Pseudomonas savastanoi pv. phaseolicola in mung bean. 【Methods】 Seven pairs of specific primers were selected to perform a series of PCR reactions on Pseudomonas savastanoi pv. phaseolicola suspensions at different concentrations,and their sensitivity was verified. 【Results】 Three pairs of primers with high sensitivity were selected. The simulated seed surface bacteria detection results showed that the minimum concentration of bacterial suspension detected by primer B4-1-F/B4-1-R and B4-2-F/B4-2-R was 1×10⁴ CFU/mL,and the minimum concentration of bacterial suspension detected by primer HB14F/HB14R was 1×10⁶ CFU/mL. The results showed that primer B4-1-F/B4-1-R and B4-2-F/B4-2-R could detect the seed sample with seed-carrying rate of 0.062 5% after shaking and washing for 1 h,and primer HB14F/HB14R could detect the seed sample with seed-carrying rate of 0.125% after shaking and washing for 4 h. 【Conclusion】 PCR could be used to rapidly detect seed carrying the Pseudomonas savastanoi pv. phaseolicola on mung bean seed surface,which was convenient and quick.

【Objective】 To screen the best hormone combination for rapid propagation of sweet potato virus-free tissue culture seedlings susceptible to sweet potato virus disease（SPVD）,and to provide technical support for the application of virus-free sweet potato seedlings in production. 【Methods】 Using Xushu 22 and Fuxushu 20 as test materials,the shoot apical meristem was cultured with varying detoxification times,virus inhibitors,combination of hormone concentration（NAA/6-BA）,subculture time and seedling cultivation. The removal rate of SPVD by different treatments was detected. The plant height of tissue cultured seedlings was measured. The survival rate of transplanting was counted. 【Results】 The removal rate of sweet potato chlorotic stunt virus（SPCSV）reached 100% in primary shoot apex meristem culture,and the removal rate of sweet potato feathery mottle virus（SPFMV）reached 100% in secondary shoot apex meristem culture. The removal rate of SPFMV stem tip pretreatment with virus inhibitor Ningnan·pyripeptide reached 100%. The optimal hormone combination for fast propagation of Xushu 22 tissue cultured seedlings was 0.05 mg/L NAA,1.0 mg/L 6-BA,and the optimal hormone combination for fast propagation of Fuxushu 20 tissue cultured seedlings was 0.01 mg/L NAA,1.0 mg/L 6-BA. The highest survival rate for Xushu 22 and Fuxushu 20 treated seedlings when subcultured for 60 days was 86.67% and 83.33%. 【Conclusion】 Secondary shoot apex meristem culture could completely eliminate SPVD. Virus inhibitor Ningnan·pyripeptide pretreatment increased the removal rate of SPFMV shoot apex. The optimal hormone combination for tissue culture seedlings for rapid propagation was 0.01-0.05 mg/L NAA,1.0 mg/L 6-BA. Refining seedlings increased the survival rate of transplanted tissue culture seedlings significantly.

Perilla frutescens（L.）Britt is one of the commonly used Chinese medicinal in China. It is one of the pharmaceutical and food homology plant resources. It has rich pharmacologically active ingredients,and has antibacterial,anti-inflammatory,antioxidant,and anti-tumor properties. This article summarized the active ingredients of different organs of Perilla frutescens（L.）Britt,reviewed terpenoids,aromatics,aliphatics,flavonoids,phenolic acids,phenylpropanoids,steroids,glycosides,and amion acids in leaves and stem,as well as main components of fatty acids and proteins in seeds. The functional and antibacterial mechanisms of each ingredient were also studied in order to provide a theoretical basis for the standardized planting and diversified application of Perilla frutescens（L.）Britt.

【Objective】 To find out the appropriate nitrogen fertilizer amount for local alfalfa planting in Togtoh County in Hohhot. 【Methods】 The agronomic traits of alfalfa under different nitrogen fertilizer levels were studied for 4 consecutive years and the economic benefit analysis was conducted based on the long-term positioning experiment using alfalfa as material with a single factor completely randomized trial design. 【Results】 The average plant height of all nitrogen treatments was higher than the control. The nitrogen fertilizer 120.00 kg/hm² had the highest average plant height（126.93 cm）. Compared with the control,the nitrogen fertilizer treatment plants had lower stem and leaf rate（1.13）. The dry-to-fresh ratio of alfalfa droped as the nitrogen application rate increased,while the dry-to-fresh ratio of the control was relatively higher（0.37）;the alfalfa hay yield reached its peak in the third year. The nitrogen fertilizer 120.00 kg/hm² had the highest total hay yield in the 4 years（96 509.24 kg/hm²）,which was 36 150.16 kg/hm² more than the control,with an enconormic yield of 74 400 yuan/hm² higher. 【Conclusion】 Nitrogen application significantly increased the plant height,hay yield and net income of alfalfa while decreasing the stem-leaf ratio. The treatment of 120.00 kg/hm² nitrogen fertilizer was the best nitrogen application rate for alfalfa planting in Togtoh County in Hohhot.