低温条件下玉米秸秆降解菌复配效果及降解特性研究

doi:10.12190/j.issn.2096-1197.2021.06.09

Abstract

Abstract: 【Objective】To combine the high-efficient compound microbes that degrade corn straw at low temperature,and to provide candidate microbial strains for corn straw returned into field in situ under promoting decay condition.【Methods】Based on the combination of corn straw degradation bacteria A3（Achromobacter deleyi strain LMG 3458）and A4（Pseudomonas plecoglossica strain NBRC）,three sets of compound microbes were prepared by supplementing functional fungi A（Aspergillus terreus）and P（Phanerochaete chrysosporium）,which were A3+A4+A,A3+A4+P,and A3+A4+A+P,respectively. The enzyme activities of the compound microbes as well as their effects on degradation rate of corn straw and relative microbial contents of single strain were studied at a low temperature of 15 ℃.【Results】 When fungi A and P were added,the relative microbial content of the bacterial combination of A3+A4 increased,and the corn straw degradation rate increased significantly（P<0.05）. The compound microbes A3+A4+A+P had the highest corn straw degradation rate of 38.79%,which was 13.04 percentage points higher than that of the bacterial combination of A3+A4. The highest FPase activity,xylanase activity,and laccase activity of the compound microbes A3+A4+A+P was observed on 30,15,and 20 day of incubation,respectively,which were 4.44 U/mL,4.00 U/mL and 60.04 U/L higher than those of the bacterial combination of A3+A4,respectively. Furthermore,the degradation rates of cellulose,hemicellulose,and lignin of the compound microbes A3+A4+A+P were higher than those of the A3+A4 by 10.77,4.15,and 21.10 percentage points,respectively.【Conclusion】The compound microbes A3+A4+A+P had higher corn straw degradation efficiency and could be used as candidate strain resources for the study and application of microbial agents for corn straw degradation.

Key words: Corn straw, Degradation rate, Compound microbes, Enzyme activity, Relative microbial content

CLC Number:

S141.4

ZHANG Bizhou, HONG Bo, ZHANG Tao, ZHAO Wantong, YU Xiaofang, GAO Julin, ZHANG Xin, Qinggeer. Compound effect of corn straw degradation microbes and their degradation characteristics at low temperature[J].Journal of Northern Agriculture, 2021, 49(6): 71-78.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2021.06.09

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Compound effect of corn straw degradation microbes and their degradation characteristics at low temperature

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