薄壳山核桃嫩枝扦插生根技术与根系发育机理研究

doi:10.12190/j.issn.2096-1197.2024.02.09

Abstract

Abstract: 【Objective】To improve the softwood cutting technology system for Carya illinoensis（Wangench.） K. Koch,and identify the endogenous substances affecting cutting root development.【Methods】Three Carya illinoensis（Wangench.） K. Koch varieties,Caddo,Jinhua and Mahan,were used as experimental materials to study the rooting rate and root effect index of cuttings under different hormone types,shading rates,humidity levels,and container conditions through four-factor four-level orthogonal test. The changes in soluble sugar content,soluble protein content,IAA content,ABA content,IAA/ABA,POD activity,and SOD activity with the root effect index were analyzed.【Results】The order of influence of various factors on the cuttings′ rooting rate of Carya illinoensis（Wangench.） K. Koch were humidity level=shading rate>types of hormone>container conditions. Cuttings under IBA treatment,0 or 60% shading rate and 60%<humidity levels≤90% could get the optimal cutting rooting rate. While cuttings under IBA treatment,60% shading rate and 60%<humidity levels of≤80% could get the optimal cuttings′ root development effect,which was within the optimal range for rooting rate screening. As the root effect index increasing,the content of soluble sugar and soluble protein in stem cuttings decreased,the IAA content and IAA/ABA increased,the ABA content decreased,SOD activity and POD activity increased. The difference in soluble protein content and POD activity between adjacent root effect index cuttings in the range of ≤0.5 were significant（P<0.05）. The difference in IAA content between adjacent root effect index cuttings reached significant levels within the range of ≥0.3（P<0.05）,and the increase in IAA/ABA continued to increase.【Conclusion】Carya illinoensis（Wangench.） K. Koch cuttings can achieve good rooting effect at IBA hormone treatment,60% shading rate and 60%<humidity level≤80%. Soluble protein,IAA content,IAA/ABA,POD activity are closely related to the root development of cuttings.

Key words: Carya illinoensis（Wangench.） K. Koch, Rooting rate, Root effect index, Nutritional indicator, Endogenous hormone, Enzyme activity

CLC Number:

S664.1

WANG Hao, LIU Wei, CAI Weijia. Study on the softwood cutting rooting technology and root development mechanism of Carya illinoensis（Wangench.） K. Koch[J].Journal of Northern Agriculture, 2024, 52(2): 79-86.

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Study on the softwood cutting rooting technology and root development mechanism of Carya illinoensis（Wangench.） K. Koch

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