Abstract: In order to provide theoretical basis and technical reference for local sheep embryo transfer industry, the synchroestrus treatment were carried out for sheep by using CIDR and Progestagen Sponge methods in the non-breeding season to make frozen embryo transfer, and effects of different estrous methods, ovarian, embryonic age, different transplantation methods and seasons on embryo transfer pregnancy rate were studied. Results showed that the sheep concentrated in estrus after synchroestrus treatment in 24-72 h, and estrus sheep were concentrated most in 36-48 h; the synchronization estrus rates of CIDR method and Progestagen Sponge method were 91.11% and 87.78% （P〉0.05）, and the synchronization estrus sheep transfer utilization rates were 82.93% and 78.48% （P 〉0.05）; one ovary containing one and two of the corpus luteum, and their pregnancy rates were 46.07% and 48.78% （P〉0.05）, the pregnancy rates of morula and blastocysts were 49.25% and 42.11% （P〉0.05）; the pregnancy rates of Laparoscopie method and operation method were 45.16% and 48.52% （P 〉0.05）; the pregnancy rates in November and December were 50.76% and 43.08% （P 〉0.05）. The frozen embryos pregnancy rate of tested sheep was 46.92%, which was basically close to Chinese frozen embryos level and lower than the fresh embryos pregnancy rate （50%-60%） of sheep in China at present. Thereby, there was no significant difference in the effects of different estrous methods, ovarian, embryonic age, different transplantation methods and seasons on embryo transfer pregnancy rate; the Progestagen Sponge could be chosen when most sheep were in estrous synchronization; as long as the quality of corpus luteum of sheep was good, embryo could be transplanted; Laparoscopie method could be widely used to release stress response of sheep and decrease the incidence rate of postoperative metrosynizesis; The frozen embryos pregnancy rate of tested sheep was lower than the fresh embryos pregnancy rate of sheep in China at present, and it could be promoted through screening high efficiency and low toxicity cryoprotectants and improve the operation level of basic technicians.