IBRV对牛单核巨噬细胞吞噬鸡红细胞功能的影响

doi:10.12160/j.issn.1672-5190.2015.04.008

畜牧与饲料科学 ›› 2015, Vol. 36 ›› Issue (4): 23-23.doi: 10.12160/j.issn.1672-5190.2015.04.008

IBRV对牛单核巨噬细胞吞噬鸡红细胞功能的影响

乌兰其其格[1] 高晶[2] 周伟光[2] 陈玉惠[2] 敖特根巴雅尔[3]

[1]内蒙古鄂托克旗农牧业局家畜改良工作站,内蒙古鄂托克旗016100 [2]内蒙古农业大学兽医学院农业部动物疾病临床诊疗技术重点实验室,内蒙古呼和浩特010018 [3]内蒙古鄂托克旗兽医局,内蒙古鄂托克旗016100

出版日期:2015-04-20 发布日期:2015-04-20
通讯作者: 乌兰其其格
作者简介:乌兰其其格（1974-），女，畜牧师．主要研究方向为家畜改良。通讯作者：敖特根巴雅尔（1974-），男，兽医师，主要研究方向为动物疫病防控。
基金资助:
国家自然科学基金项目（30860202）.

Effect of IBRV on Phagocytizing Chicken Erythrocytes Function of Bovine Monocyte-macrophage

Wulanqiqige, GAO Jing, ZHOU Wei-guang, CHEN Yu-hui, Aotegenbayaer （1.Livestock Improvement Station, Agricultural and Animal Husbandry Bureau of Etuoke County of Inner Mongolia, Etuoke County 016100,China;2.Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease of Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University,Hohhot 010018,China;3.Veterinary Bureau of Etuoke County, Etuoke County 016100,China）

Online:2015-04-20 Published:2015-04-20

摘要/Abstract

摘要： 为研究牛传染性鼻气管炎病毒（IBRV）感染对牛单核巨噬细胞吞噬功能的影响，应用淋巴细胞分离液获得牛外周血单个核细胞，通过贴壁培养成为单核巨噬细胞。 IBRV 感染单核巨噬细胞24 h后加入鸡红细胞，测定被感染细胞对鸡红细胞的吞噬率。结果显示，单核巨噬细胞被IBRV 感染后，对鸡红细胞的吞噬率显著降低（P<0.05）。

Abstract: To investigate the effects of infectious bovine rhinotracheitis virus （IBRV） infection on phagocytizing chicken erythrocytes function of bovine monocyte-macrophage, lymphocyte separating medium was used to isolate the bovine peripheral blood mononuclear cells. The isolated cells were subsequently cultured to monocyte-macrophage by adherent culture method. The chicken erythrocytes were added to bovine monocyte-macrophage which was infected by IBRV for 24 h, and the phagocytizing chicken erythrocytes rate of the infected cells was determined. The results showed that the phagocytic chicken erythrocytes rate of IBRV infected bovine monocyte-macrophage was significantly reduced （P〈0.05）.

中图分类号:

S858.235.3 S852.42

乌兰其其格,高晶,周伟光,陈玉惠,敖特根巴雅尔. IBRV对牛单核巨噬细胞吞噬鸡红细胞功能的影响[J]. 畜牧与饲料科学, 2015, 36(4): 23-23.

IBRV对牛单核巨噬细胞吞噬鸡红细胞功能的影响

Effect of IBRV on Phagocytizing Chicken Erythrocytes Function of Bovine Monocyte-macrophage

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