3株非典型羊源布鲁菌的基因分型研究

doi:10.12160/j.issn.1672-5190.2019.12.026

畜牧与饲料科学 ›› 2019, Vol. 40 ›› Issue (12): 108-112.doi: 10.12160/j.issn.1672-5190.2019.12.026

• 疾病防治 • 上一篇

3株非典型羊源布鲁菌的基因分型研究

沈利平¹, 王正廷², 刘志国³

1.内蒙古乌兰察布市凉城县蛮汉镇人民政府,内蒙古凉城 013750;
2.内蒙古乌兰察布市凉城县农牧和科技局,内蒙古凉城 013750;
3.内蒙古自治区综合疾病预防控制中心,内蒙古呼和浩特 010031

收稿日期:2019-10-17 出版日期:2019-12-30 发布日期:2020-03-05
通讯作者: 刘志国(1985—),男,副主任技师,博士,主要研究方向为布病防控研究。
作者简介:沈利平(1984—),男,畜牧师,主要研究方向为畜牧与扶贫开发。
基金资助:
内蒙古自治区自然科学基金项目(2018MS08004)

Characterization of the Genotype of Three Clinically Isolated Atypical Brucella Strains from Sheep

SHEN Li-ping¹, WANG Zheng-ting², LIU Zhi-guo³

1.People's Government of Manhan Town of Liangcheng County of Ulanqab City of Inner Mongolia, Liangcheng 013750, China;
2.Agriculture, Animal Husbandry and Science and Technology Bureau of Liangcheng County of Ulanqab City of Inner Mongolia, Liangcheng 013750, China;
3.Inner Mongolia Center for Comprehensive Disease Prevention and Control, Hohhot 010031, China

Received:2019-10-17 Online:2019-12-30 Published:2020-03-05

摘要/Abstract

摘要： [目的]探讨采用基因分型方法对非典型布鲁菌鉴定的实用性,为非典型菌株的鉴别提供参考。[方法]采用常规鉴定方法和VITEK 2.0全自动细菌鉴定分析系统对菌株进行初步鉴定,利用AMOS-PCR进行种型鉴定,应用多位点可变数目串联重复序列分析方法(MLVA)确定菌株的基因型。[结果]常规鉴定结果显示试验菌株为疑似布鲁菌;VITEK 2.0全自动细菌鉴定系统显示3株试验菌株均为布鲁菌;AMOS-PCR扩增表明试验菌株均为羊种菌,MLVA聚类分析表明试验菌株与羊种2型布鲁菌紧密地聚为一类,属东地中海基因型,并在Panel 2B中发现了新的基因型(4-4-3-7-5),命名为CN2B-45。[结论]MLVA基因分型方法对非典型布鲁菌具有极高的分辨力,是非典型布鲁菌分型鉴别的最佳策略。

关键词: 非典型布鲁菌, MLVA, 基因分型

Abstract: [Objective]To verify the practicability of genotyping method for the confirmation of atypical Brucella strains, and to provide reference for the discriminative identification of the atypical ones. [Method]The both conventional identification methods and VITEK 2. 0 automatic bacteria identification analysis system were used to carry out the preliminary identification of the clinical Brucella strains from sheep. AMOS-PCR was applied to discriminate the species of the strains. The genotype of the tested strains were determined by using MLVA assay. [Result]Conventional identification results showed that the three tested isolates were Brucella-suspected strains; VITEK 2. 0 automatic bacteria identification analysis system confirmed that the tested strains all belonged to Brucella genus; AMOS-PCR amplification showed that the strains were Brucella melitensis(B. melitensis); MLVA cluster analysis revealed that the tested strains were close with B. melitensis biovar 2 and belonged to Eastern Mediterranean lineages; a new genotype (4-4-3-7-5) was found in the Panel 2B and was designated as CN2B-45. [Conclusion]MLVA assay has excellent discriminative power for atypical Brucella strain and is the best strategy for identification of atypical Brucella strain.

Key words: atypical Brucella strain, MLVA, genotyping

中图分类号:

沈利平, 王正廷, 刘志国. 3株非典型羊源布鲁菌的基因分型研究[J]. 畜牧与饲料科学, 2019, 40(12): 108-112.

SHEN Li-ping, WANG Zheng-ting, LIU Zhi-guo. Characterization of the Genotype of Three Clinically Isolated Atypical Brucella Strains from Sheep[J]. Animal Husbandry and Feed Science, 2019, 40(12): 108-112.

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3株非典型羊源布鲁菌的基因分型研究

Characterization of the Genotype of Three Clinically Isolated Atypical Brucella Strains from Sheep

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