去势对公犬血清抗氧化指标的影响

doi:10.12160/j.issn.1672-5190.2021.05.020

畜牧与饲料科学 ›› 2021, Vol. 42 ›› Issue (5): 120-123.doi: 10.12160/j.issn.1672-5190.2021.05.020

去势对公犬血清抗氧化指标的影响

姜西迪, 阮崇美, 刘灿, 徐艳丽, 蒲越, 熊永洁, 李静

安徽科技学院动物科学学院,安徽凤阳 233100

收稿日期:2021-05-08 出版日期:2021-09-30 发布日期:2021-11-25
通讯作者: 李静（1979—）,女,副教授,博士,主要研究方向为中草药防治动物疾病。
作者简介:姜西迪（1996—）,女,硕士研究生,主要研究方向为兽医学。
基金资助:
安徽省大学生创新创业训练项目（2018S10879015）; 安徽省高校协同创新项目（GXXT-2019-013）

Effects of Castration on Serum Antioxidant Indexes in Male Dogs

JIANG Xi-di, RUAN Chong-mei, LIU Can, XU Yan-li, PU Yue, XIONG Yong-jie, LI Jing

College of Animal Science,Anhui Science and Technology University,Fengyang 233100,China

Received:2021-05-08 Online:2021-09-30 Published:2021-11-25

摘要/Abstract

摘要： [目的] 研究去势对公犬血清抗氧化指标的影响。[方法] 选择12只健康1岁龄左右的雄性中华田园犬,随机分成对照组和去势组,每组6只,饲养5 d后开始进行试验。对两组犬抽血,对去势组进行睾丸摘除术,对照组不做手术。之后两组分别采集术后第5、10和15天的血液,将所有血液离心获取血清,检测血清中的超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性,以及过氧化氢（H₂O₂）和丙二醛（MDA）含量,分析两组之间4项指标的差异显著性。[结果] 对照组和去势组的SOD活性无显著差异（P>0.05）,去势组的GSH-Px活性在去势后第5、10和15天低于对照组,且两组差异显著（P<0.05）。去势组去势后第10天和第15天的MDA和H₂O₂含量均极显著（P<0.01）高于对照组。[结论] 去势对公犬血清SOD活性无明显影响,可导致公犬血清GSH-Px活性显著降低,MDA和H₂O₂含量显著升高。

关键词: 公犬, 去势, 过氧化氢, 超氧化物歧化酶, 谷胱甘肽过氧化物酶, 丙二醛

Abstract: [Objective] To investigate the effects of castration on serum antioxidant indexes in male dogs. [Method] A total of 12 healthy male Chinese rural dogs aged around one year were randomly assigned into a control group or a castration group with 6 dogs per group. The formal experiment was performed after a 5-day pre-feeding period. At the initial of the experiment, blood samples of the experimental dogs were collected. The testectomy was performed in the dogs in castration group, and the dogs in control group received no testectomy. The blood samples of the experimental dogs were collected on 5^th, 10^th and 15^th days after testectomy, and the serums were obtained by centrifugation. The activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） as well as the contents of hydrogen peroxide （H₂O₂） and malonaldehyde （MDA） in serums of the dogs in castration group and control group were determined and statistically compared. [Result] No significant （P>0.05） differences in serum SOD activities between control group and castration group were observed. On 5^th, 10^th and 15^th days after testectomy, the serum GSH-Px activities of castration group were significantly （P<0.05） lower than those of control group. Castration group had extremely significantly （P<0.01） higher serum contents of MDA and H₂O₂ on 10^th and 15^th days after testectomy compared with control group. [Conclusion] Castration has no significant effect on the serum SOD activities of male dogs, but significantly reduces the serum GSH-Px activities and elevates the serum contents of MDA and H₂O₂.

Key words: male dogs, castration, hydrogen peroxide, superoxide dismutase, glutathione peroxidase, malonaldehyde

中图分类号:

Q493.5

姜西迪, 阮崇美, 刘灿, 徐艳丽, 蒲越, 熊永洁, 李静. 去势对公犬血清抗氧化指标的影响[J]. 畜牧与饲料科学, 2021, 42(5): 120-123.

JIANG Xi-di, RUAN Chong-mei, LIU Can, XU Yan-li, PU Yue, XIONG Yong-jie, LI Jing. Effects of Castration on Serum Antioxidant Indexes in Male Dogs[J]. Animal Husbandry and Feed Science, 2021, 42(5): 120-123.

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去势对公犬血清抗氧化指标的影响

Effects of Castration on Serum Antioxidant Indexes in Male Dogs

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