Abstract: The research aimed to determine the polysaccharide content in medicinal plant Acanthopanax senticosus and provide scientific basis for the quality control and resource development of A. senticosus. Taking glucose as control, the polysaccharide content in A. senticosus was determined by using phenol-sulfuric acid method. The results showed that the standard curve of glucose was A=0.0133C-0.0025（R2=0.997 5）. The polysaccharide content was 2.40%. The method had good precision, and the adsorption of reaction products was stable within 2 hours, and the average recovery was 99.1%.