Abstract: In the present study, five seasonal regulation of grazing intensity treatments and one control treatment were set to assess the response of soil physical and chemical properties to seasonal regulation of grazing intensity in Stipa breviflora steppe. The results revealed that grazing had obvious impact on soil physical properties： the soil bulk density was significantly increased with the increase of grazing intensity （P〈0.05）, especially in the whole-year heavily grazed soil; the soil moisture was decreased with the increase of grazing intensity and the statistically significant reduction was observed in 0-20 cm layer of the whole-year heavily grazed soil （P〈0.05）. Different grazing treatments had no significant impact on soil organic matter content of 0-20 cm and 30-40 cm soil layer （P〉0.05）, and had no significant impact on soil total nitrogen and available nitrogen content of 10-d0 cm soil layer （P〉0.05）; for the treatment of no grazing in spring ＋ heavy grazing in summer ＋ moderate grazing in autumn, the soil available nitrogen content of 0-10 cm soil layer was significantly increased （P〈0.05）; the total phosphorus content of 10-20 cm soil layer in the above mentioned treatment was significantly increased （P〈0.05）, but no significant differences in soil total phosphorus content in the other soil layers between the different grazing treatments were observed （P〉0.05）; grazing had no significant impact on soil available phosphorus and total potassium content （P〉0.05）, and had no significant impact on soil available potassium content of 0-20 cm soil layer （P〉0.05）. The combined data demonstrate that the grazing intensity seasonal regulation mode of no grazing in spring ＋ heavy grazing in summer ＋ moderate grazing in autumn is the optimum utilization pattern of Stipa breviflora steppe.