Abstract: The aim of the present study was to establish a high performance liquid chromatography （HPLC） method for determination of tetrahydropalmatine in the Chinese medicine compound Jianwei dispersible tablet, and to evaluate and compare the tetrahydropalmatine content in the dispersible tablet prepared by mixed extraction method and single extraction method using the established HPLC method. The HPLC system was equipped with the Agilent SB-C18 column （250 mm × 4.6 mm, 5 μm）; the methanol-phosphate （65：35） buffer （pH value = 6.0） was used as the mobile phase; the isolated gradients were detected at 280 nm wavelength with an ultraviolet detection. The results showed that the established HPLC method had a good linear relationship in the range of 0.1-1.0 μg/mL for tetrahydropalmatine （r=0.9989）; the average recovery of tetrahydropalmatine was 99.99% with RSD of 0.91%; the average content of tetrahydropalmatine in the dispersible tablet prepared by mixed extraction method and single extraction method was 2.430 7 mg/g （RSD=0.57%, n=6） and 1.937 5 mg/g （RSD=2.17%, n=6） respectively, indicating that the preparation effect of the former method is better. The results obtained in this study demonstrated that the established HPLC method is sensitive, convenient, accurate and reproducible for determination of tetrahydropalmatine in Chinese medicine compound Jianwei dispersible tablet.