畜牧与饲料科学 ›› 2018, Vol. 39 ›› Issue (9): 19-23.doi: 10.12160/j.issn.1672-5190.2018.09.004

• 著者文摘 • 上一篇    下一篇

滩羊尾脂组织候选基因mRNA表达量分析研究

马丽娜;马青;李颖康   

  1. 宁夏农林科学院动物科学研究所,宁夏银川750002
  • 收稿日期:2018-08-01 出版日期:2018-08-19 发布日期:2019-08-19
  • 通讯作者: 马丽娜-宁夏农林科学院动物科学研究所,宁夏银川750002
  • 作者简介:马而娜(1985-),女,助理研究员,硕士,主要研究方向为动物遗传育种。;通讯作者:马青(1973-),男,副研究员,硕士,主要研究方向为绵羊育种。
  • 基金资助:
    宁夏自然科学基金项目(NZl6099);宁夏回族自治区农业育种专项(2013NYYZ04).

mRNA Expression of the Candidate Genes Associated with Tail Fat Deposition in Tail Fat Tissue of Tan Sheep

MA Li-na[1];MA Qing[1];LI Ying-kang[1]   

  1. Institute of Animal Science,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002,China
  • Received:2018-08-01 Online:2018-08-19 Published:2019-08-19

摘要: 为进一步筛选与滩羊脂肪沉积相关的候选基因,对利用基因芯片扫描和选择信号检测得到的滩羊尾脂组织中与脂肪合成代谢相关的6个重要候选基因(PDGFD、CAVI、PPARG、HSL、PLA2G16、RETN)进行mRNA表达量研究。结果表明,6个基因在滩羊尾脂中均有表达,其中,HSL基因的mRNA表达量显著高于其他基因(P〈0.05)。研究结果为滩羊尾型选育及品种改良提供了参考。

关键词: 滩羊;尾脂;mRNA表达;Real-time;PCR

Abstract: In an attempt to further screen the candidate genes associated with tail fat deposition of Tan sheep, the mRNA expression of six candidate genes (PDGFD, CAV1, PPARG, HSL, PLA2G16, RETN) screened by genechip scanning and selection signatures detection was quantitatively assessed. The mRNA expression of the six genes were simultaneously observed in the tail fat tissue of Tan sheep, and the significantly higher mRNA expression was found in HSL gene (P〈0.05). The results obtained in this study provide valuable references for tail type selection and variety improvement of Tan sheep.

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