超高效液相色谱—串联质谱法检测鸡血液样品中喹诺酮类药物残留

doi:10.12160/j.issn.1672-5190.2019.11.003

畜牧与饲料科学 ›› 2019, Vol. 40 ›› Issue (11): 13-16.doi: 10.12160/j.issn.1672-5190.2019.11.003

超高效液相色谱—串联质谱法检测鸡血液样品中喹诺酮类药物残留

刘瑜, 毕孝瑞, 张彤, 姜玲玲, 金雁, 徐宜宏, 李晓东, 耿庆华

沈阳海关技术中心,辽宁沈阳 110016

收稿日期:2019-09-19 出版日期:2019-11-30 发布日期:2019-12-19
通讯作者: 耿庆华（1977—）,女,研究员,博士,主要从事动物及其产品检验检疫工作。
作者简介:刘瑜（1982—）,女,高级工程师,硕士,主要从事农、兽药残留检测工作。
基金资助:
辽宁省自然科学基金计划重点项目“畜禽体液中兽药多残留快速筛查方法研究及与可食组织中残留相关性研究”（20170520089）

Determination of Quinolones Residues in Chicken Blood Samples by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

LIU Yu, BI Xiao-rui, ZHANG Tong, JIANG Ling-ling, JIN Yan, XU Yi-hong, LI Xiao-dong, GENG Qing-hua

Shenyang Customs Technology Center,Shenyang 110016,China

Received:2019-09-19 Online:2019-11-30 Published:2019-12-19

摘要/Abstract

摘要： 旨在建立鸡血液样品中多种喹诺酮类药物残留的超高效液相色谱—串联质谱方法。样品经乙腈提取,经Waters Acquity UPLC BEH C₁₈色谱柱分离,以甲醇和0.1%甲酸水溶液为流动相进行梯度洗脱,电喷雾正离子（ESI⁺）模式电离,MRM扫描模式检测。经方法学验证,该方法的线性关系、回收率、精密度均符合要求。应用超高效液相色谱—串联质谱法建立了鸡血液样品中多种喹诺酮类药物同时检测的方法,实现了动物血液样品中12种喹诺酮类药物同时定性、定量分析。

关键词: 鸡血液, 喹诺酮类药物, 超高效液相色谱—串联质谱;

Abstract: The objective of this study was to establish an ultra performance liquid chromatography-tandem mass spectrometry method for the determination of various quinolones residues in chicken blood samples. The samples were extracted with acetonitrile solution and were separated by Waters Acquity UPLC system with a BEH C₁₈ column. The used mobile phases were methanol and 0.1% formic acid aqueous solution in gradient elution. The electrospray was operated in the positive ionization mode, and the compounds were identified by MRM mode. Methodological identification showed that the linear relationship, recovery and precision of the established method met the requirements. A method for simultaneous determination of various quinolones residues in chicken blood samples by ultra performance liquid chromatography-tandem mass spectrometry was successfully established, and qualitative and quantitative analysis of 12 quinolones residues was achieved simultaneously.

Key words: chicken blood, quinolones, ultra performance liquid chromatography-tandem mass spectrometry

中图分类号:

S859.84

刘瑜, 毕孝瑞, 张彤, 姜玲玲, 金雁, 徐宜宏, 李晓东, 耿庆华. 超高效液相色谱—串联质谱法检测鸡血液样品中喹诺酮类药物残留[J]. 畜牧与饲料科学, 2019, 40(11): 13-16.

LIU Yu, BI Xiao-rui, ZHANG Tong, JIANG Ling-ling, JIN Yan, XU Yi-hong, LI Xiao-dong, GENG Qing-hua. Determination of Quinolones Residues in Chicken Blood Samples by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Animal Husbandry and Feed Science, 2019, 40(11): 13-16.

参考文献 10

[1]	孙泽祥,鲍伟华,杨挺,等.克伦特罗在猪尿液和血液中残留消除相关性研究[J].中国畜牧兽医,2010,37(8):160-163.
[2]	伍华雯,郭平,张远芳,等.高效液相色谱—串联质谱法同时测定供港生猪尿液中29种限用兽药残留[J].分析测试学报,2016,35(10):1261-1266.
[3]	付体鹏,张峰,刘力,等.高效液相色谱—电喷雾—iFunnel—串联质谱法同时测定猪尿液中20种禁用兽药残留[J].分析测试学报,2013,32(10):1153-1159.
[4]	刘荟岭. HPLC法测定禽血清和肌肉中恩诺沙星含量的方法改进与应用[D].哈尔滨:东北农业大学,2013.
[5]	李桂芳. 土霉素和磺胺嘧啶在猪尿液和可食性组织中残留相关性研究[D].武汉:华中农业大学,2009.
[6]	郭亚文,卜晓娜,刘楚君.动物源性食品中氟喹诺酮类药物残留色谱质谱检测技术研究进展[J].中国兽药杂志,2019,53(1):64-76.
[7]	陈颖,白欣,宋月.超高效液相色谱—三重四级杆串联质谱法测定多种动物源性食品中11种喹诺酮类药物残留[J].中国卫生检验杂志,2018,28(14):1680-1683.
[8]	梁素丹,陈剑刚,张艳.全自动固相萃取—超高效液相色谱—串联质谱法同时测定动物肌肉中喹诺酮类和四环素类兽药残留[J].中国食品卫生杂志,2018,30(2):151-157.
[9]	高何刚,王瑞,杜赛,等.超高效液相色谱—串联质谱联用法同时测定鸡肉中磺胺和喹诺酮类兽药残留[J].中国卫生检验杂志,2017,27(17):2481-2485.
[10]	黄坤,吴婉琴,罗彤,等.QuEChERS—超高效液相色谱—串联质谱法同时测定畜禽肉中11种喹诺酮类兽药残留[J].肉类研究,2019,33(3):40-45.

超高效液相色谱—串联质谱法检测鸡血液样品中喹诺酮类药物残留

Determination of Quinolones Residues in Chicken Blood Samples by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

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