腺垂体细胞促性腺激素分泌及其体外培养研究进展

doi:10.12160/j.issn.1672-5190.2023.05.010

畜牧与饲料科学 ›› 2023, Vol. 44 ›› Issue (5): 71-76.doi: 10.12160/j.issn.1672-5190.2023.05.010

腺垂体细胞促性腺激素分泌及其体外培养研究进展

李泽廷¹,苏少锋¹,乔健敏¹,马跃军¹,孙培培²,赵俊利¹,吴海青¹

1.内蒙古自治区农牧业科学院，内蒙古呼和浩特 010031
2.内蒙古草原同源牧业有限公司，内蒙古呼和浩特 010020

收稿日期:2022-07-07 修回日期:2023-06-20 出版日期:2023-09-30 发布日期:2023-11-14
通讯作者: 吴海青（1983—），男，副研究员，博士，主要研究方向为马属动物繁育及应用技术。
作者简介:李泽廷（1994—），女，硕士，主要研究方向为马属动物繁殖机理。
基金资助:
内蒙古农牧业创新基金项目“牛羊品种育繁技术研究与示范——基于GEBV技术的乳用驴核心群建立及选育技术研究”(2022CXJJM02);内蒙古自治区呼和浩特市重大科技项目“驴高效繁殖技术研究与示范”(2020-农-重-2);中央引导地方科技发展基金项目“驴定时配种及配套技术集成与示范”(2021ZY0001)

Research Progress in Gonadotropin Secretion and in vitro Culture of Anterior Pituitary Cells

LI Zeting¹,SU Shaofeng¹,QIAO Jianmin¹,MA Yuejun¹,SUN Peipei²,ZHAO Junli¹,WU Haiqing¹

1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences，Hohhot 010031，China
2. Inner Mongolia Caoyuan Tongyuan Animal Husbandry Co.，Ltd.，Hohhot 010020，China

Received:2022-07-07 Revised:2023-06-20 Online:2023-09-30 Published:2023-11-14

摘要/Abstract

摘要：

下丘脑-垂体-性腺轴是控制动物性激素分泌的主要内分泌系统。围绕该系统开展深入研究对于提高家畜繁殖性能及治疗繁殖疾病等方面具有重要意义。下丘脑调控促性腺激素释放激素（gonadotropin-releasing hormone，GnRH）的分泌，该激素又进一步调控腺垂体，促进其分泌促卵泡素（follicle stimulating hormone，FSH）和促黄体素（luteinizing hormone，LH）。建立腺垂体细胞体外培养体系可为揭示腺垂体激素分泌机理的研究提供方法学基础。介绍了腺垂体在脑室中所处的位置以及腺垂体细胞的类型和主要分泌的激素，综述了腺垂体细胞的分离培养和鉴定方法，以及外源物质对体外培养腺垂体细胞分泌促性腺激素（gonadotropin，Gn）影响的研究进展，以期为深入研究下丘脑-垂体-卵巢轴的功能及机制，优化与完善腺垂体细胞体外培养体系，筛选Gn分泌调节药物提供参考。

关键词: 垂体, 腺垂体细胞, 促性腺激素, 激素分泌

Abstract:

The hypothalamic-pituitary-gonadal axis is the primary endocrine system that regulates the secretion of sex hormones in animals. In-depth research on this system is crucial for improving livestock reproductive performance and treating reproductive diseases. The hypothalamus regulates the secretion of gonadotropin-releasing hormone （GnRH）, which in turn regulates the anterior pituitary, promoting its secretion of follicle stimulating hormone （FSH） and luteinizing hormone （LH）. Establishing an in vitro culture system for anterior pituitary cells can provide a methodological basis for unveiling the mechanism of anterior pituitary hormone secretion. This article introduces the anatomic location of anterior pituitary in brain ventricle as well as the types of anterior pituitary cells and the primary hormones secreted by them, reviews the isolation, culture and identification methods of anterior pituitary cells, as well as the research progress in the effects of exogenous substances on the secretion of gonadotropin （Gn） by anterior pituitary cells in vitro, in hoping to better understand the function and mechanism of the hypothalamic-pituitary-ovarian axis, optimize and improve the in vitro culture system of anterior pituitary cells, and provide references for screening Gn secretion regulating drugs.

Key words: pituitary gland, anterior pituitary cell, gonadotropin, hormone secretion

中图分类号:

S814.1

李泽廷, 苏少锋, 乔健敏, 马跃军, 孙培培, 赵俊利, 吴海青. 腺垂体细胞促性腺激素分泌及其体外培养研究进展[J]. 畜牧与饲料科学, 2023, 44(5): 71-76.

LI Zeting, SU Shaofeng, QIAO Jianmin, MA Yuejun, SUN Peipei, ZHAO Junli, WU Haiqing. Research Progress in Gonadotropin Secretion and in vitro Culture of Anterior Pituitary Cells[J]. Animal Husbandry and Feed Science, 2023, 44(5): 71-76.

参考文献 34

[1]	MRUK D D, CHENG C Y. The mammalian blood-testis barrier: Its biology and regulation[J]. Endocrine Reviews, 2015, 36(5): 564-591. doi: 10.1210/er.2014-1101 pmid: 26357922
[2]	SHARPE R M. Sperm counts and fertility in men: A rocky road ahead[J]. EMBO Reports, 2012, 13(5): 398-403. doi: 10.1038/embor.2012.50
[3]	RUWANPURA S M, MCLACHLAN R I, MEACHEM S J. Hormonal regulation of male germ cell development[J]. The Journal of Endocrinology, 2010, 205(2): 117-131. doi: 10.1677/JOE-10-0025
[4]	RUWANPURA S M, MCLACHLAN R I, STANTON P G, et al. Pathways involved in testicular germ cell apoptosis in immature rats after FSH suppression[J]. The Journal of Endocrinology, 2008, 197(1): 35-43. doi: 10.1677/JOE-07-0637
[5]	ABEL M H, WOOTTON A N, WILKINS V, et al. The effect of a null mutation in the follicle-stimulating hormone receptor gene on mouse reproduction[J]. Endocrinology, 2000, 141(5): 1795-1803. doi: 10.1210/endo.141.5.7456 pmid: 10803590
[6]	KUMAR T R, WANG Y, LU N F, et al. Follicle stimulating hormone is required forovarian follicle maturation but not male fertility[J]. Nature Genetics, 1997, 15(2): 201-204. doi: 10.1038/ng0297-201
[7]	NAVEED M R, AHMAD N, AHMAD I, et al. Effects of FSH extracted from in vitro cultured anterior pituitary cells of male buffalo calves on body and testes weight, serum FSH and total cholesterol and hematological variables in male rabbits[J]. Animal Reproduction Science, 2014, 150(3/4): 125-129. doi: 10.1016/j.anireprosci.2014.09.009
[8]	崔海燕, 秦达念. 睾丸内生精细胞凋亡与激素调控[J]. 汕头大学医学院学报, 2005, 18(1): 57-59.
[9]	KARAYAZI A Ö, GOVINDRAJAN N, LOPETEGUI-GONZALEZ I, et al. Prolactin: A hormone with diverse functions from mammary gland development to cancer metastasis[J]. Seminars in Cell and Developmental Biology, 2021, 114:159-170. doi: 10.1016/j.semcdb.2020.10.005 pmid: 33109441
[10]	WILLIAMS V L, DEGUZMAN A, DANG H, et al. Common and specific effects of the two major forms of prolactin in the rat testis[J]. American Journal of Physiology Endocrinology and Metabolism, 2007, 293(6): 1795-1803.
[11]	PETERSEN C, SODER O. The sertoli cell: A hormonal target and ′super′ nurse for germ cells that determines testicular size[J]. Hormone Research, 2006, 66(4): 153-161.
[12]	CANUL-MEDINA G, FERNANDEZ-MEJIA C. Morphological,hormonal, and molecular changes in different maternal tissues during lactation and post-lactation[J]. The Journal of Physiological Sciences, 2019, 69(6): 825-835. doi: 10.1007/s12576-019-00714-4
[13]	SUNTORNSARATOON P, WONGDEE K, GOSWAMI S, et al. Bone modeling in bromocriptine-treated pregnant and lactating rats: Possible osteoregulatory role of prolactin in lactation[J]. American Journal of Physiology Endocrinology and Metabolism, 2010, 299(3): 426-436.
[14]	郑慧媛, 王兰, 苏军龙, 等. 大鼠腺垂体细胞的体外培养和鉴定[J]. 陕西医学杂志, 2010, 39(6): 643-645.
[15]	杨地, 吕文, 郭伟, 等. 培养鼠腺垂体细胞的形态和分泌功能实验研究[J]. 现代临床医学生物工程学杂志, 2004(2): 91-93.
[16]	蒋小飞, 周惠芳, 周伯如, 等. SD大鼠腺垂体细胞体外分离方法的比较[J]. 医学研究生学报, 2017, 30(12): 1272-1276.
[17]	尹慧勇, 赵晓娥, 马保华. 山羊腺垂体细胞的分离培养与鉴定[J]. 西北农林科技大学学报(自然科学版), 2010, 38(1): 11-16.
[18]	史继新, 刘承基. 脑垂体细胞培养的研究[J]. 金陵医院学报, 1995, 8(1): 35-38.
[19]	LIU P C, LIU K, LIU J F, et al. Transfection of the IHH gene into rabbit BMSCs in a simulated microgravity environment promotes chondrogenic differentiation and inhibits cartilage aging[J]. Oncotarget, 2016, 7(39): 62873-62885. doi: 10.18632/oncotarget.v7i39
[20]	JHA R, WU Q L, SINGH M, et al. Simulated microgravity and 3D culture enhance induction, viability, proliferation and differentiation of cardiac progenitors from human pluripotent stem cells[J]. Scientific Reports, 2016, 6:30956. doi: 10.1038/srep30956 pmid: 27492371
[21]	WANG Y J, ZHANG Y P, ZHANG S C, et al. Rotating microgravity-bioreactor cultivation enhances the hepatic differentiation of mouse embryonic stem cells on biodegradable polymer scaffolds[J]. Tissue Engineering Part A, 2012, 18(21/22): 2376-2385. doi: 10.1089/ten.tea.2012.0097
[22]	宁泽乾. 拟微重力三维培养体系在腺垂体来源细胞培养中的应用研究[D]. 广州: 广东药科大学, 2020.
[23]	王文华. GnRH类似物通过miR-488调控牛促卵泡素分泌的研究[D]. 长春: 吉林大学, 2020.
[24]	黄荷凤. 瘦素对生殖功能的影响[J]. 浙江医学, 2002, 24(4): 198-199.
[25]	齐长学, 付世新, 张丽, 等. 瘦素(leptin)与雌性哺乳动物的繁殖活动关系的研究进展[J]. 中国奶牛, 2008(10): 38-40.
[26]	SIAWRYS G, GAJEWSKA A. In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig[J]. Polish Journal of Veterinary Sciences, 2017, 20(1): 67-76. doi: 10.1515/pjvs-2017-0010
[27]	BARB C R, BARRETT J B, KRAELING R R. Role of leptin in modulating the hypothalamic-pituitary axis and luteinizing hormone secretion in the prepuberal gilt[J]. Domestic Animal Endocrinology, 2004, 26(3): 201-214. pmid: 15036375
[28]	刘贝, 周惠芳, 周伯如, 等. 补肾助孕方对大鼠垂体细胞促性腺激素的调节[J]. 医学研究生学报, 2018, 31(7): 703-708.
[29]	秦向锋. E₂和PrRPs对草鱼垂体促性腺激素的调节作用及其分子机制[D]. 武汉: 华中农业大学, 2019.
[30]	AHMED A E, JIN J, HASHIZUME T. Effects of methyl-beta-cyclodextrin on the release of luteinizing hormone, follicle stimulating hormone, prolactin and growth hormone from cultured bovine anterior pituitary cells[J]. Journal of Experimental and Applied Animal Sciences, 2017, 2(2): 102. doi: 10.20454/jeaas.2017.1230
[31]	刘立文, 李寸欣, 马云, 等. 蜂花粉对公鸡外周血浆促性腺激素以及体外培养垂体细胞释放促性腺激素的影响[J]. 中国家禽, 2016, 38(15): 60-62.
[32]	BELLMANN A, SCHNEIDER F, KANITZ W, et al. Effect of GnRH and its antagonist (Antarelix) on LH release from cultured bovine anterior pituitary cells[J]. Acta Veterinaria Hungarica, 2002, 50(1): 79-92. pmid: 12061239
[33]	张凌齐. 脂联素对皖南花猪垂体生殖内分泌的影响[D]. 合肥: 安徽农业大学, 2013.
[34]	喻晶华, 郭建, 郭景, 等. β-内啡肽对体外灌流大鼠垂体前叶细胞LH分泌的抑制作用[J]. 生殖医学杂志, 1992, 1(1): 31-33,63.

腺垂体细胞促性腺激素分泌及其体外培养研究进展

Research Progress in Gonadotropin Secretion and in vitro Culture of Anterior Pituitary Cells

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 34

相关文章 13

编辑推荐

Metrics

本文评价

[1]	李泽廷, 苏少锋, 赵俊利, 孙培陪, 白晋宇, 吴海青. GnRH的生物学作用及其类似物在马属动物中的应用研究进展[J]. 畜牧与饲料科学, 2023, 44(6): 82-87.
[2]	唐斯嘎, 韩乌兰图雅, 娜仁花, 马跃军, 赵俊利, 吴海青. 人绒毛膜促性腺激素对母驴促排卵效果的研究[J]. 畜牧与饲料科学, 2021, 42(3): 59-65.
[3]	邢雪静, 尹国安. GnRH类似物疫苗免疫去势效果的影响因素[J]. 畜牧与饲料科学, 2019, 40(11): 61-64.
[4]	张永昌,娜仁花,达拉吉如嘎,刘永斌,吴海青. 非繁殖季节母驴诱导发情、排卵效果的研究[J]. 畜牧与饲料科学, 2018, 39(9): 10-18.
[5]	徐元青,王建林,邵宝平. 促性腺激素释放激素（GnRH）结构及调控的研究进展[J]. 畜牧与饲料科学, 2014, 35(12): 31-31.
[6]	张文才. 不同激素对母犬的诱导发情和产仔效果的研究[J]. 畜牧与饲料科学, 2013, 34(6): 20-20.
[7]	吴师,李华慧,覃现才,宾娟容. 人绒毛膜促性腺激素（HCG）对杂交黄牛、水牛受胎率的影响[J]. 畜牧与饲料科学, 2011, 32(2): 66-66.
[8]	丁淑春,王艳芬,姜波,汪连云,马洪梅. 母牛受配率低的原因及对策[J]. 畜牧与饲料科学, 2008, 29(3): 118-118.
[9]	栾庆江,张锁链,仓明. 不同来源的促性腺激素对奶牛超数排卵的影响[J]. 畜牧与饲料科学, 2003, 24(6): 53-53.
[10]	滕勇. GnRH、PGF2α诱导同期发情效果的比较[J]. 畜牧与饲料科学, 2002, 23(4): 1-1.
[11]	邸怀忠,丁涛. 家畜应激状态下下丘脑—垂体—肾上腺轴和交感神经系统的功能[J]. 畜牧与饲料科学, 1996, 17(1): 31-31.
[12]	侯勇跃,梅欣. 山羊遗传性甲状腺肿甲状腺脑下垂体的超微结构研究[J]. 畜牧与饲料科学, 1992, 13(1): 5-5.
[13]	王念功,马群山. 猪胚胎移植技术的研究概况[J]. 畜牧与饲料科学, 1989, 10(4): 23-23.