布鲁氏菌内蒙古分离株omp31基因的克隆与序列分析

doi:10.12160/j.issn.1672-5190.2009.10.006

畜牧与饲料科学 ›› 2009, Vol. 30 ›› Issue (10): 15-15.doi: 10.12160/j.issn.1672-5190.2009.10.006

布鲁氏菌内蒙古分离株omp31基因的克隆与序列分析

陆静[1] 关平原[1] 乌日罕[2] 特木尔巴根[2] 马立峰[2] 菊花[2]

[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [2]内蒙古动物疫病预防与控制中心,内蒙古呼和浩特010010

出版日期:2009-10-20 发布日期:2009-10-20
通讯作者: 陆静
作者简介:陆静（1981-），女，硕士，主要研究方向为动物传染病学通讯作者：关平原（1961-），男，教授，博士，主要研究方向为动物传染病的诊断与防制．E-mail：gpynm@yahoo．com.cn

Cloning and Sequence Analysis of omp31 Genes of Brucella Strain Isolated from Inner Mongolia

LU Jing, GUAN Ping-yuan, Wurihan, Temuerbagen, MA Li-feng, Juhua （1.College of Animal Science and Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China; 2.Central of Animal Disease Prevention and Control of Inner Mongolia, Hohhot 010010, China）

Online:2009-10-20 Published:2009-10-20

摘要/Abstract

摘要： 对三株布鲁氏菌内蒙古分离株的omp31基因进行克隆与序列分析。参照GenBank中布鲁氏菌的omp31基因序列，设计一对特异性引物，用PCR方法扩增布鲁氏菌omp31基因，对PCR产物进行克隆、测序，将测定序列拼接后与GenBank中获得的参考毒株omp31基因序列进行了比较。结果显示，三株布鲁氏菌分离株的omp31基因开放阅读框核苷酸序列同源性均为100．0％；与Brucella ceti B14／94、Brucella neotomae 5K33、Brucella suis 1330三个参考毒株omp31基因核苷酸序列同源性最高，为100．0％；与Brucella cartisRM6／66、Brucella melitensis 183、Brucella melitensis 16M三个参考毒株omp31基因核苷酸序列同源性为99．9％；与Brucella melitensisRevl参考毒株omp31基因核苷酸序列同源性为99．6％；与Brucella ovis ATCC25840参考毒株omp31基因核苷酸序列同源性最低．为98．8％、

Abstract: Sequence analysis was conducted on genes of omp31 from 3 different Brucella strain isolated from Inner Mongolia. A pair of primers were designed for amplification of Brucella omp31 genes according to the reported of Brucella omp31 genes complete sequence. omp31 genes were amplified from 3 different Brucella strains by polymerase chain reaction （PCR）, and the PCR products were cloned into pMDI9-T vector and sequences were analyzed. The results showed nucleotide sequences in homology among 3 different Brucella isolates omp31 genes were 100.0 %. Nucleotide sequence in homology was 100.0 % between the three isolates genes and Brucella suis 1330, Brucella ceti B14/94 and Brucella neotomae 5K33, its homology was 99.0 % compared with omp31 gene of Brucella canis RM6/ 66, Brucella melitensis 183 and Brucella melitensis 16M, but the homology between the obtained and Brucella ovis ATCC25840 was the lowest, only 98.8 %.

中图分类号:

R516.7

陆静,关平原,乌日罕,特木尔巴根,马立峰,菊花. 布鲁氏菌内蒙古分离株omp31基因的克隆与序列分析[J]. 畜牧与饲料科学, 2009, 30(10): 15-15.

布鲁氏菌内蒙古分离株omp31基因的克隆与序列分析

Cloning and Sequence Analysis of omp31 Genes of Brucella Strain Isolated from Inner Mongolia

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