Abstract: According to the $2 gene sequence of Avian reovirus strain 176 （ARV 176） published in GenBank, two pairs of primers were synthesized. The total RNA of ARV isolate T-98 and C-98 isolated in Tianjin and Inner Mongolia were extracted. The $2 gene of virus was amplified by RT-PCR and then cloned into pESAY-T1 plasmid and sequenced. The results showed that the $2 genes of ARV C-98 and ARV T-98 isolates were 1 324 bp, included 15 bp 5＇ non-coding region and 58 bp 3＇ non-coding region, and contained one ORF（ 16-1 266）. Compared nucleotide sequence showed that high homology was 99.8% between ARV T-98 and ARV C-98. The homology of $2 genes of ARV C-98 and ARV T-98 isolates was 99.2%-99.8% comparing with S2 genes of reference strains ARV 176, ARV 918, ARV 919, ARV 1733, ARV GX2010, ARV S1133 and DRV YJL, and was 92.1% -94.1% comparing with $2 genes of reference strains ARV 138 and ARV AVS-B, and was 87.3%-87.8% comparing with $2 genes of reference strains ARV 601SI, ARV 916 and ARV 1017-1, and was 77.3%-78.2% comparing with S2 genes of reference strains DRV 091,DRV S!2 and DRV S14, and was 2.4%-5.5% comparing with S2 genes of reference strains MRV 3, MRV 3-jin- 1 and MRV 3-T3D. Phylogenetic analysis indicated that S2 genes of ARV T-98 and C-98 and 20 ARV strains were separated into four lineages.