Abstract: The combined application of question guiding teaching model and traditional teaching model in teaching of Food Microbiology is of significance for the cultivation of undergraduates＇ ability to autonomously learn, solve practical problems and innovate. The traditional teaching model makes undergraduates to systemically learn theoretical knowledge after helping them to construct a knowledge system, which brings a lot of information for undergraduates in classroom. The question guiding teaching model is favorable to stimulate the undergraduates＇ interest in learning professional knowledge, and to improve the undergraduates＇ ability to consult document, analyze and solve problems, and to cooperate. Based on the contents of Food Microbiology, the two teaching models are compared and are comprehensively applied in teaching, and the existing problems in teaching of Food Microbiology are discussed, so as to provide bases for the better and extensive application of question guiding teaching model combining with traditional teaching model.